Intranasal delivery of nanomicelle curcumin promotes corneal epithelial wound healing in streptozotocin-induced diabetic mice.
Intranasal delivery of nanomicelle curcumin promotes corneal epithelial wound healing in streptozotocin-induced diabetic mice.
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纳米胶束姜黄素鼻内给药促进链脲佐菌素诱导的糖尿病小鼠角膜上皮伤口愈合
DOI：
10.1038/srep29753
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发表时间：
2016-07-11
影响因子：
4.6
通讯作者：
Wu X
中科院分区：
文献类型：
--
作者：
Guo C;Li M;Qi X;Lin G;Cui F;Li F;Wu X
角膜神经主要来源于三叉神经节（TG）的眼分支。糖尿病性角膜病变中角膜神经病变导致上皮退行性改变。有效的药物输送到TG可能有利于糖尿病角膜病变的治疗。本文描述了鼻内递送纳米胶束姜黄素以纠正TG中的病理生理条件，从而促进链脲佐菌素诱导的糖尿病小鼠中的角膜上皮/神经伤口愈合。建立糖尿病小鼠角膜上皮损伤模型。进行眼部局部和/或鼻内纳米胶束姜黄素治疗，并探索治疗功效和作用机制。结果表明，姜黄素纳米胶束鼻内给药可促进角膜上皮创伤愈合和角膜感觉恢复。糖尿病小鼠角膜上皮损伤后，角膜和TG神经元中活性氧积累增加，自由基清除剂减少，炎性细胞因子mRNA表达增加，神经营养因子mRNA表达减少。鼻内纳米胶束姜黄素治疗有效地恢复了这些病理生理条件，特别是TG神经元的病理生理条件，并且用眼部局部联合鼻内治疗观察到了加强的恢复。这些发现表明，鼻内姜黄素治疗有效地帮助促进糖尿病角膜上皮/神经伤口愈合。这种新的治疗方法可能是糖尿病性角膜病变的一种有前途的强化治疗方法。
Corneal nerves are mainly derived from the ophthalmic branch of the trigeminal ganglion (TG). Corneal neuropathy contributes to epithelial degenerative changes in diabetic keratopathy. Efficient drug delivery to TG may be beneficial for the treatment of diabetic keratopathy. This article described intranasal delivery of nanomicelle curcumin to correct pathophysiological conditions in TG to promote corneal epithelial/nerve wound healing in streptozotocin-induced diabetic mice. A diabetic mice model with corneal epithelium abrasion was established. Ocular topical and/or intranasal nanomicelle curcumin treatments were performed and treatment efficacy and mechanisms of action were explored. Results showed that intranasal nanomicelle curcumin treatment promoted corneal epithelial wound healing and recovery of corneal sensation. Enhanced accumulation of reactive oxygen species, reduced free radical scavengers, increased mRNA expressions of inflammatory cytokines and decreased mRNA expressions of neurotrophic factors in the cornea and TG neuron were observed in diabetic mice with corneal epithelium abrasions. Intranasal nanomicelle curcumin treatment effectively recovered these pathophysiological conditions, especially that of the TG neuron and a strengthened recovery was observed with ocular topical combined with intranasal treatment. These findings indicated that intranasal curcumin treatment effectively helped promote diabetic corneal epithelial/nerve wound healing. This novel treatment might be a promising strengthened therapy for diabetic keratopathy.