Microparticles shed from different antigen-presenting cells display an individual pattern of surface molecules and a distinct potential of allogeneic T-cell activation
Microparticles shed from different antigen-presenting cells display an individual pattern of surface molecules and a distinct potential of allogeneic T-cell activation
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DOI：
10.1111/j.1365-3083.2005.01551.x
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发表时间：
2005-03-01
影响因子：
3.7
通讯作者：
Herrmann, M
中科院分区：
文献类型：
--
作者：
Kolowos, K;Gaipl, US;Herrmann, M
各种细胞如血小板、淋巴细胞、内皮细胞、红细胞和单核细胞释放表面衍生微粒（mps）。我们分析了从培养的抗原呈递人细胞（APC）和人细胞系的上清液中分离的mp。通过动态光散射进行的粒度测定显示，在活细胞中，颗粒的特征尺寸范围为80 nm至300 nm，在辐照细胞中，颗粒的特征尺寸范围为400 nm至1200 nm。采用流式细胞术，我们观察到部分改变的表面蛋白组成的mp相比，他们的细胞来源。MP来源于树突状细胞（DC）不同，从单核细胞和单核细胞衍生的巨噬细胞释放的表面组成。在功能测定中，这些mp刺激同种异体反应性T细胞。用UV-B或脂多糖处理细胞强烈影响MP的数量、免疫刺激特性和表面组成。凋亡巨噬细胞的MP能够降低活性巨噬细胞的刺激能力，但不能降低DC的刺激能力。另一方面，来自DC的凋亡MP总是刺激性的。这是第一份关于DC释放MP的研究报告，并与其他APC释放的MP进行了比较。
Various cells such as platelets, lymphocytes, endothelial cells, red blood cells and monocytes do release surface-derived microparticles (mps). We analysed mp isolated from supernatants of cultured antigen-presenting human cells (APCs) and human cell lines. Particle sizing by dynamic light scattering revealed a characteristic size of the particles ranging from 80 nm to 300 rum in viable cells and from 400 nm to 1200 nm in irradiated cells. Employing flow-cytometry, we observed partly an altered surface protein composition of the mp compared to their cellular source. Mp originating from dendritic cells (DCs) differed in their surface composition from those released from monocytes and monocyte-derived macrophages. In functional assays, these mp stimulated alloreactive T-cells. The treatment of the cells with either UV-B or lipopolysaccharide strongly influenced the quantity, the immunostimulatory features and the surface composition of the mp. Mp from apoptotic macrophages were able to reduce the stimulatory capacity of vital macrophages but not of DC. Apoptotic mps from DC, on the other hand, were always stimulatory. This is the first report regarding the study of mp released from DC and compared with those released from other APC.