DNA/polyethyleneimine/hyaluronic acid small complex particles and tumor suppression in mice
DNA/polyethyleneimine/hyaluronic acid small complex particles and tumor suppression in mice
复制标题
DOI：
10.1016/j.biomaterials.2009.12.032
复制
发表时间：
2010-04-01
期刊：
影响因子：
14
通讯作者：
Koyama, Yoshiyuki
中科院分区：
文献类型：
--
作者：
Ito, Tomoko;Yoshihara, Chieko;Koyama, Yoshiyuki
非病毒载体有效体内基因转染的最高障碍是（1）与生物分子的非特异性相互作用，和（2）大尺寸的DNA复合物颗粒。透明质酸（HA）对DNA/聚乙烯亚胺（PEI）复合物的保护涂层有效地减少了与生物分子的不利相互作用。在这里，我们发现HA也保护DNA/PEI复合物通过冷冻干燥和再水化过程防止聚集和失活。它使我们能够制备浓缩的非常小的DNA复合物颗粒（
The highest barriers for non-viral vectors to an efficient in vivo gene transfection would be (1) nonspecific interaction with biological molecules, and (2) large size of the DNA complex particles. Protective coating of the DNA/polyethyleneimine (PEI) complexes by hyaluronic acid (HA) effectively diminished the adverse interactions with biological molecules. Here we found HA also protected the DNA/PEI complexes against aggregation and inactivation through lyophilization-and-rehydration procedures. It allows us to prepare the concentrated very small DNA complex particles (