Heat shock protein profiles on the protein and gene expression levels in olive flounder kidney infected with Streptococcus parauberis
Heat shock protein profiles on the protein and gene expression levels in olive flounder kidney infected with Streptococcus parauberis
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DOI：
10.1016/j.fsi.2013.03.355
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发表时间：
2013-06-01
影响因子：
4.7
通讯作者：
Jung, Tae Sung
中科院分区：
文献类型：
--
作者：
Cha, In Seok;Kwon, Joseph;Jung, Tae Sung
热休克蛋白（HSPs）已经在细胞暴露于各种应激，包括感染性病原体。本研究采用无标记、定量蛋白质组学方法和转录基因表达分析研究了橄榄比目鱼全肾感染相关热休克蛋白及其编码基因。在副金黄色葡萄球菌感染牙鲆的过程中，诱导编码Hsp10、Hsp40A4、Hsp40B6、Hsp40B11、Hsp60、Hsp70、葡萄糖调节蛋白78 （Grp78）、Hsp90 α、Hsp90 β和Grp94的基因，Hsp60、Hsp70、Hsp90 α、Hsp90 β和Grp94的蛋白水平随时间的变化而发生差异。随后的结果还显示，Hsp60、Hsp70、Hsp90 α、Hsp90 β和Grp94是橄榄比目鱼细菌感染过程中的优势和关键热休克蛋白。这是首次估计热休克蛋白在橄榄比目鱼暴露于细菌感染的免疫反应中的差异参与。(C) 2013 Elsevier Ltd.版权所有。
Heat shock proteins (HSPs) have been observed in cells exposed to a variety of stresses, including infectious pathogens. This study used a label-free, quantitative proteomic approach and transcriptional gene expression analysis to investigate infection-related HSP proteins and their encoding genes in whole kidneys from olive flounder (Paralichthys olivaceus). During Streptococcus parauberis infection in the flounder, the genes encoding Hsp10, Hsp40A4, Hsp40B6, Hsp40B11, Hsp60, Hsp70, glucose regulated protein 78 (Grp78), Hsp90 alpha, Hsp90 beta and Grp94 were induced, and the protein levels of Hsp60, Hsp70, Hsp90 alpha, Hsp90 beta and Grp94 were differentially regulated over time. Subsequent results also revealed that Hsp60, Hsp70, Hsp90 alpha, Hsp90 beta and Grp94 appear to be the dominant and critical HSPs in olive flounder during bacterial infection. This is the first estimation of the differential involvement of HSPs in the immune response of olive flounder exposed to bacterial infection. (C) 2013 Elsevier Ltd. All rights reserved.