De novo expression of the cell adhesion molecule E-selectin on gastric cancer endothelium
De novo expression of the cell adhesion molecule E-selectin on gastric cancer endothelium
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DOI：
10.1007/s004230050096
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发表时间：
1998-03-01
影响因子：
2.3
通讯作者：
Schildberg, FW
中科院分区：
文献类型：
--
作者：
Mayer, B;Spatz, H;Schildberg, FW
背景和目的：血管生成和肿瘤脉管系统的分子表型决定肿瘤的生长和转移。患者/方法：在 58 名胃癌患者中，使用免疫组织化学比较了正常、发炎和恶性胃组织中的血管密度和内皮细胞的抗原谱。结果：在良性胃粘膜和原发性胃癌中，血管密度与炎症无关。然而，原发性肿瘤中血管分布的增加与高肿瘤细胞密度呈正相关，表明肿瘤诱导的血管生成（P=0.00001）。 P-选择素在大多数胃粘膜样本的一小部分血管中表达，并且在存在中度至强白细胞浸润的情况下增加。 VCAM-1 阳性粘膜血管很少见，且与炎症无关。正常和发炎的内皮细胞中不存在定义出芽血管的 E-选择素和 EN 7/44 抗原。相反，在原发性胃癌中观察到E-选择素和EN 7/44抗原的从头表达。 E-选择素阳性血管优先发现于血管丰富的肿瘤区域（P=0.0043），与白细胞浸润无关。肿瘤相关内皮细胞上VCAM-1的上调与炎症密切相关(P=0.019)，而P-选择素的表达与良性粘膜相似。结论：差异表达的血管分子可能影响外渗白细胞的功能特征，并代表抗胃癌治疗的新靶点。
Background and aims: Angiogenesis and the molecular phenotype of the tumor vasculature determine tumor growth and metastasis. Patients/Methods: In a series of 58 gastric cancer patients, vascular density and the antigenic profile of endothelial cells in normal, inflamed and malignant gastric tissues were compared using immunohistochemistry. Results: In both benign gastric mucosa and primary gastric cancer vascular density was inflammation-independent. However, increased vascularity in primary tumors was positively associated with a high tumor cell density suggesting tumor-induced angiogenesis (P=0.00001). P-selectin was expressed in most of the gastric mucosa samples on a small fraction of vessels and increased in the presence of moderate to strong leukocyte infiltrate. VCAM-1 positive mucosal vessels were rare and showed no association with inflammation. E-selectin and the EN 7/44 antigen defining budding vessels were absent on normal and inflamed endothelium. In contrast, in primary gastric cancer de novo expression of both E-selectin and the EN 7/44 antigen was observed. E-selectin positive vessels were preferentially found in vascular-rich tumor areas (P=0.0043) independently of leukocyte infiltration. Upregulation of VCAM-1 on tumor-associated endothelium was closely related to inflammation (P=0.019), while P-selectin expression resembled that in benign mucosa. Conclusions: Differentially expressed vascular molecules may influence the functional characteristics of extravasating leukocytes and represent new targets in anti-gastric cancer therapy.