Noninvasive assessment of changes in cytochrome-c oxidase oxidation in human subjects during visual stimulation
Noninvasive assessment of changes in cytochrome-c oxidase oxidation in human subjects during visual stimulation
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DOI：
10.1097/00004647-199906000-00002
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发表时间：
1999-06-01
影响因子：
6.3
通讯作者：
Villringer, A
中科院分区：
文献类型：
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作者：
Heekeren, HR;Kohl, M;Villringer, A
在这项研究中，作者使用了全光谱近红外光谱法来非侵入性地评估视觉刺激期间枕叶皮质中血红蛋白氧合和细胞色素c氧化酶氧化还原状态（Cyt-Ox）的变化。系统使用白色光源（卤素灯）。从受试者头部反射的光通过光谱仪进行光谱解析，并分散在冷却的电荷耦合器件相机上。作者使用这种方法表明：（1）可以定量评估视觉刺激期间人类枕叶皮层中脑血红蛋白氧合的变化（氧合血红蛋白浓度增加，脱氧血红蛋白浓度降低）。(2)功能激活期间的光谱变化不能完全由血红蛋白氧合变化单独解释;分析中必须包括Cyt-Ox。只有考虑到Cyt-Ox，才能解释大脑活动增加引起的光谱变化。(3)人类受试者枕叶皮层中的细胞色素c氧化酶在视觉刺激期间被瞬时氧化。这使我们能够同时测量血管和细胞内能量状态。
In this study the authors used a whole-spectrum near-infrared spectroscopy approach to noninvasively assess changes in hemoglobin oxygenation and cytochrorne-c oxidase redox state (Cyt-Ox) in the occipital cortex during visual stimulation. The system uses a white light sourer (halogen lamp). The light reflected from the subject's head is spectrally resolved by a spectrograph and dispersed on a cooled charge-coupled device camera. The authors showed the following using this approach: (1) Changes in cerebral hemoglobin oxygenation (increase in concentration of oxygenated hemoglobin, decrease in concentration of deoxygenated hemoglobin) in the human occipital cortex during visual stimulation can be assessed quantitatively. (2) The spectral changes during functional activation cannot be completely explained by changes in hemoglobin oxygenation solely; Cyt-Ox has to be included in the analysis. Only if Cyt-Ox is considered can the spectral changes in response to increased brain activity be explained. (3) Cytochrome-c oxidase in the occipital cortex of human subjects is transiently oxidized during visual stimulation. This allows us to measure vascular and intracellular energy status simultaneously.