Isolation and characterization of two DREB1 genes encoding dehydration-responsive element binding proteins in chicory (Cichorium intybus)
Isolation and characterization of two DREB1 genes encoding dehydration-responsive element binding proteins in chicory (Cichorium intybus)
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菊苣 (Cichorium intybus) 中编码脱水响应元件结合蛋白的两个 DREB1 基因的分离和表征
DOI：
10.1007/s10725-013-9866-8
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发表时间：
2014-05-01
影响因子：
4.2
通讯作者：
Zhao, Gengmao
中科院分区：
文献类型：
--
作者：
Liang, Mingxiang;Chen, Dandan;Zhao, Gengmao
从菊苣（Cichorium intybus）中克隆出两个新的DREB（脱水响应元件结合蛋白）基因，分别命名为CiDREB1A和CiDREB1B。这两个基因都包含一个保守的EREBP/AP2结构域，并且基于系统发育分析被归类到DREB亚家族的A - 1亚组。实时定量PCR分析表明，低温而非ABA极大地诱导了这两个CiDREB1基因的表达，这表明这些基因参与不依赖ABA的胁迫信号通路。亚细胞定位分析显示，两个CiDREB都定位在细胞核。此外，我们通过酵母单杂交分析表明，这两种CiDREB蛋白与RD19A的DRE基序结合。这些结果表明，CiDREB1A和CiDREB1B是菊苣中胁迫响应信号的重要调节因子。
Two novel DREB (dehydration-responsive element-binding protein) genes, designated as CiDREB1A and CiDREB1B, were cloned from chicory (Cichorium intybus). Both of these genes contained a conserved EREBP/AP2 domain and were classified into the A-1 subgroup of the DREB subfamily based on phylogenetic analysis. Quantitative real-time PCR analysis revealed that low temperature, but not ABA, greatly induced the expression of both CiDREB1 genes, suggesting that these genes are involved in ABA-independent stress signaling pathways. A subcellular localization assay revealed that both CiDREBs localized to the nucleus. In addition, we showed by yeast one-hybrid analysis that these two CiDREB proteins bind to the DRE motif of RD19A. These results suggest that CiDREB1A and CiDREB1B are important regulators of stress-responsive signaling in chicory.