Mitogen-activated protein kinase (MAP kinase), MAP kinase kinase and c-Mos stimulate glucose transport in Xenopus oocytes.
Mitogen-activated protein kinase (MAP kinase), MAP kinase kinase and c-Mos stimulate glucose transport in Xenopus oocytes.
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丝裂原激活蛋白激酶（MAP 激酶）、MAP 激酶激酶和 c-Mos 刺激非洲爪蟾卵母细胞中的葡萄糖转运。
DOI：
10.1042/bj2950351
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
G. Gould
中科院分区：
文献类型：
--
作者：
N. W. Merrall;R. Plevin;D. Stokoe;P. Cohen;A. Nebreda;G. Gould
有丝分裂原和生长因子强烈地刺激所有细胞中的葡萄糖运输，为它们的生长和分裂提供能量，但对这些激动剂促进糖吸收的信号机制知之甚少。在这里，我们发现，当有丝分裂原激活的蛋白激酶(MAP)被显微注射到非洲爪哇卵母细胞中时，脱氧葡萄糖和3-O-甲基葡萄糖向这些卵母细胞的转运被刺激了约2.5倍。我们还证明，显微注射原癌基因产物c-mos(一种激活非洲爪哇卵母细胞中MAP激酶的MAP激酶激活剂)和纯化的MAP激酶在脱氧葡萄糖转运方面产生类似的增加。由于MAP激酶的激活是对几乎所有有丝分裂原和生长因子的普遍反应，我们认为其下游效应之一是刺激葡萄糖运输活性。
Mitogens and growth factors acutely stimulate glucose transport in all cells to supply energy for their growth and division, but little is known about the signalling mechanism by which these agonists promote sugar uptake. Here we show that the transport of deoxyglucose and 3-O-methylglucose into Xenopus laevis oocytes is stimulated about 2.5-fold when mitogen-activated protein kinase (MAP kinase) is microinjected into these oocytes. We also demonstrate that microinjection of the proto-oncogene product c-Mos (an activator of MAP kinase kinase, which activates MAP kinase in Xenopus oocytes), and purified MAP kinase kinase produce similar increases in deoxyglucose transport. Since the activation of MAP kinase is a general response to almost all mitogens and growth factors, we propose that one of its downstream effects is the stimulation of glucose-transport activity.