Functional association between the nef gene product and gag‐pol region of HIV‐1
Functional association between the nef gene product and gag‐pol region of HIV‐1
复制标题
DOI：
10.1016/s0014-5793(99)01791-3
复制
发表时间：
2000-01
期刊：
影响因子：
3.5
通讯作者：
T. Ono;Y. Iwatani;A. Nishimura;A. Ishimoto;H. Sakai
中科院分区：
文献类型：
--
作者：
T. Ono;Y. Iwatani;A. Nishimura;A. Ishimoto;H. Sakai
Nef基因在灵长类免疫缺陷病毒分离株中的功能是多样的。我们发现nefmutation对两个HIV-1克隆NL 432和LAI之间的病毒复制有不同的影响。NL 432的Nef功能与LAI相似，但NL 432的Nef突变对侵染力的影响更大。对NL 432和LAI之间的一系列嵌合病毒的分析表明，gag-polregion负责nefmutation的差异效应。Nef与gag-pol之间的功能关联表明Nef的潜在靶点之一位于gag-pol区域内。
Nef gene function is diverse among virus isolates of primate immunodeficiency viruses. We found differential effects ofnefmutation on the virus replication between two HIV‐1 clones, NL432 and LAI. Thenefmutation in NL432 affected the infectivity more severely compared with that in LAI, although the Nef functions of both clones were comparable. Analysis with a series of chimeric viruses between NL432 and LAI revealed that thegag‐polregion was responsible for the differential effect ofnefmutation. The functional association between Nef andgag‐polsuggested that one of the potential targets of Nef was located within thegag‐polregion.