Effect of the insect phenoloxidase on the metabolism of L-DOPA
Effect of the insect phenoloxidase on the metabolism of L-DOPA
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昆虫酚氧化酶对左旋多巴代谢的影响
DOI：
10.1002/arch.21457
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发表时间：
2018-08-01
影响因子：
2.2
通讯作者：
Huang, Wuren
中科院分区：
文献类型：
--
作者：
Wu, Kai;Han, Fang;Huang, Wuren
昆虫酚氧化酶原（PPO）诱导病原体周围的黑化。在黑化之前，PPO被丝氨酸蛋白酶切割成酚氧化酶（PO）。昆虫PPO也可以被病原体分泌的外源蛋白酶以及其他化合物如乙醇和氯化十六烷基吡啶（CPC）激活。然而，这些激活剂对PO活性的影响尚不清楚。在本研究中，昆虫内源性丝氨酸蛋白酶AMM 1，α-糜蛋白酶。和乙醇激活重组果蝇PPO 1（rPPO 1），PO活性因激活剂的不同而不同。在黑化过程中PO诱导的中间体也有显着变化，其数量和丰度。因此，本研究表明，PPO的活化机制影响PO活性。这也表明PO诱导的不同中间产物可能由于其毒性而影响黑化过程中的抗菌活性。
Insect prophenoloxidase (PPO) induces melanization around pathogens. Before melanization, PPO is cleaved into phenoloxidase (PO) by serine proteases. Insect PPO can also be activated by exogenous proteases secreted by pathogens as well as by other compounds, such as ethanol and cetylpyridinium chloride (CPC). However, the effect of these activators on the activity of PO is unclear. In this study, the insect endogenous serine protease AMM1, alpha-chymotrypsin. and ethanol were used to activate recombinant Drosophila PPO1 (rPPO1), and the PO activity differed depending on the activator applied. The PO-induced intermediates during melanization also varied markedly in their numbers and abundances. Therefore, this study indicates that the mechanism of PPO activation influences PO activity. It also suggests that PO-induced different intermediates may affect the antibacterial activity during melanization due to their toxicity.