Structural Changes and Astrocyte Response of the Lateral Geniculate Nucleus in a Ferret Model of Ocular Hypertension
Structural Changes and Astrocyte Response of the Lateral Geniculate Nucleus in a Ferret Model of Ocular Hypertension
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DOI：
10.3390/ijms21041339
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Aihara, Makoto
中科院分区：
文献类型：
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作者：
Fujishiro, Takashi;Honjo, Megumi;Aihara, Makoto
我们研究了雪貂高眼压模型外侧膝状体（LGN）的结构变化和星形胶质细胞反应。在10只雪貂中，通过将培养的结膜细胞注射到右眼前房中诱导OH; 6只正常雪貂用作对照。用霍乱毒素B进行的顺行轴突追踪显示，在接受OH眼投射的LGN层中，萎缩性损伤是明显的。免疫组化分析与NeuN，胶质细胞酸性蛋白（GFAP），和Iba-1的抗体进行特异性标记的神经元，星形胶质细胞和小胶质细胞在LGN。在接受OH眼投射的LGN层中，NeuN免疫反应性显著降低，GFAP和Iba-1免疫反应性显著增加。有趣的是，LGN层之间的免疫反应性的变化是显着不同的。C层显示出比A和A1层更严重的损伤。还观察到LGN中的继发性退行性变化，包括每个LGN层中的神经元损伤和星形胶质细胞反应。这些结果表明，我们的白鼬模型的OH是有价值的研究过程中的视网膜-脑传输的视觉通路在青光眼的损害。C层的脆弱性首次被揭示出来。
We investigated structural changes and astrocyte responses of the lateral geniculate nucleus (LGN) in a ferret model of ocular hypertension (OH). In 10 ferrets, OH was induced via the injection of cultured conjunctival cells into the anterior chamber of the right eye; six normal ferrets were used as controls. Anterograde axonal tracing with cholera toxin B revealed that atrophic damage was evident in the LGN layers receiving projections from OH eyes. Immunohistochemical analysis with antibodies against NeuN, glial fibrillary acidic protein (GFAP), and Iba-1 was performed to specifically label neurons, astrocytes, and microglia in the LGN. Significantly decreased NeuN immunoreactivity and increased GFAP and Iba-1 immunoreactivities were observed in the LGN layers receiving projections from OH eyes. Interestingly, the changes in the immunoreactivities were significantly different among the LGN layers. The C layers showed more severe damage than the A and A1 layers. Secondary degenerative changes in the LGN were also observed, including neuronal damage and astrocyte reactions in each LGN layer. These results suggest that our ferret model of OH is valuable for investigating damages during the retina-brain transmission of the visual pathway in glaucoma. The vulnerability of the C layers was revealed for the first time.