Tick-borne flavivirus infection in Ixodes scapularis larvae:: Development of a novel method for synchronous viral infection of ticks
Tick-borne flavivirus infection in Ixodes scapularis larvae:: Development of a novel method for synchronous viral infection of ticks
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DOI：
10.1016/j.virol.2007.03.057
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发表时间：
2007-09-01
期刊：
影响因子：
3.7
通讯作者：
Bloom, Marshall E.
中科院分区：
文献类型：
--
作者：
Mitzel, Dana N.;Wolfinbarger, James B.;Bloom, Marshall E.
被受感染的蜱叮咬后，蜱传黄病毒会引起人类脑炎、脑膜炎和出血热。虽然这些病毒大部分时间都在蜱虫中，但对病毒-载体相互作用知之甚少。本文报道了一种用Langat病毒（LGTV）同步感染肩突硬蜱幼虫的简便方法。这种技术导致大约96%的蜱虫被感染。LGTV感染和复制通过病毒抗原表达和病毒RNA积累来证明。此外，蜱虫将LGTV传播给100%的小鼠，并通过蜕皮将病毒维持到下一个生命阶段。该技术通过模拟自然感染途径和通过使用减毒病毒株感染蜱来规避当前方法中存在的局限性，从而使该技术成为研究病毒和蜱复制、发病机制和传播的决定因素的有力工具。爱思唯尔公司出版
Following a bite from an infected tick, tick-borne flaviviruses cause encephalitis, meningitis and hemorrhagic fever in humans. Although these viruses spend most of their time in the tick, little is known regarding the virus-vector interactions. We developed a simple method for synchronously infecting Ixodes scapularis larvae with Langat virus (LGTV) by immersion in media containing the vir-us. This technique resulted in approximately 96% of ticks becoming infected. LGTV infection and replication were demonstrated by both viral antigen expression and the accumulation of viral RNA. Furthermore, ticks transmitted LGTV to 100% of the mice and maintained the virus through molting into the next life stage. This technique circumvents limitations present in the current methods by mimicking the natural route of infection and by using attenuated virus strains to infect ticks, thereby making this technique a powerful tool to study both virus and tick determinants of replication, pathogenesis and transmission. Published by Elsevier Inc.