Maturation of carbonic anhydrase IV expression in rabbit kidney.
Maturation of carbonic anhydrase IV expression in rabbit kidney.
复制标题
兔肾中碳酸酐酶 IV 表达的成熟。
DOI：
10.1152/ajprenal.2001.280.5.f895
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Schwartz,GJ
中科院分区：
文献类型：
--
作者：
Winkler,CA;Kittelberger,AM;Watkins,RH;Maniscalco,WM;Schwartz,GJ
Carbonic anhydrase (CA) IV facilitates renal acidification by catalyzing the dehydration of luminal H2CO3. CA IV is expressed in proximal tubules, medullary collecting ducts, and A-intercalated cells of the mature rabbit kidney (Schwartz GJ, Kittelberger AM, Barnhart DA, and Vijayakumar S.Am J Physiol278: F894–F904, 2000). In view of the maturation of HCOtransport in the proximal tubule and collecting duct, the ontogeny of CA IV expression was examined. During the first 2 wk, CA IV mRNA was expressed in maturing cortex and medulla at ∼20% of adult levels. The maturational increase was gradual in cortex over 3–5 wk of age but surged in the medulla, so that mRNA levels appeared higher than those in the adult medulla. In situ hybridization showed very little CA IV mRNA at 5 days, with increases in deep cortex and medullary collecting ducts by 21 days. Expression of CA IV protein in the cortex and medulla was minimal at 3 days of age but then apparent in the juxtamedullary region, A-intercalated cells and medullary collecting ducts by 18 days; there was little labeling of the proximal straight tubules of the medullary rays. Thus CA IV expression may be regulated to accommodate the maturational increase in HCOabsorption in the proximal tubule. In the medullary collecting duct, there is a more robust maturation of CA IV mRNA and protein, commensurate with the high rate of HCOabsorption in the neonatal segment.
