Evidence That More than 90% of beta-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus.
Evidence That More than 90% of beta-Glucuronidase-Expressing Cells after Particle Bombardment Directly Receive the Foreign Gene in their Nucleus.
复制标题
证据%20那%20更多%20比%2090%%20的%20β-葡萄糖醛酸酶表达%20细胞%20后%20粒子%20轰击%20直接%20接收%20%20外来%20基因%20在%20他们的%20细胞核中。
作者：
Toshiya Yamashita;A. Iida;H. Morikawa
将携带β-葡萄糖醛酸酶（GUS）基因的质粒DNA包被在金颗粒上，导入培养的烟草（Nicotiana tabacum L.）cv Bright Yellow-2）细胞。细胞学分析的细胞内位置的引入的金颗粒之前和之后的GUS表达测定表明，超过90%的GUS表达细胞轰击后，在其细胞核中收到的DNA包被的颗粒。
Plasmid DNA harboring the beta-glucuronidase (GUS) gene, coated on gold particles, was delivered into cultured tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cells using a pneumatic particle gun. Cytological analyses of intracellular location of the introduced gold particles before and after GUS expression assay indicated that more than 90% of GUS-expressing cells after bombardment received a DNA-coated particle in their nucleus.