Bacterial heavy metal transporter MerC increases mercury accumulation in Arabidopsis thaliana
Bacterial heavy metal transporter MerC increases mercury accumulation in Arabidopsis thaliana
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DOI：
10.1016/j.bej.2012.11.007
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发表时间：
2013-02-15
影响因子：
3.9
通讯作者：
Pan-Hou, Hidemitsu
中科院分区：
文献类型：
--
作者：
Kiyono, Masako;Oka, Yumiko;Pan-Hou, Hidemitsu
本研究评估了转基因拟南芥表达细菌重金属转运蛋白 MerC 用于汞污染植物修复的可行性。使用抗 MerC 抗体免疫染色方法发现，MerC、MerC-SYP121 或 MerC-AtVAM3 蛋白在转基因植物的叶段中表达。通过蔗糖密度梯度离心和免疫印迹分析，发现MerC、MerC-SYP121和MerC-AtVAM3分别定位于高尔基体、质膜和液泡膜。表达 merC-SYP121 的转基因拟南芥植物对汞的抵抗力更强，并且比野生型拟南芥积累了更多的汞。这些结果表明，细菌重金属转运蛋白MerC的表达促进了转基因拟南芥中汞的转运和积累，这可能是改良植物修复汞污染的有效方法。 (C) 2012 Elsevier B.V. 保留所有权利。
This study evaluated the feasibility of transgenic Arabidopsis engineered to express the bacterial heavy metal transporter MerC for the phytoremediation of mercury pollution. MerC, MerC-SYP121, or MerC-AtVAM3 proteins were found to be expressed in leaf segments of transgenic plants using an anti-MerC antibody immunostaining method. By sucrose density gradient centrifugation and immunoblotting analyses, MerC, MerC-SYP121, and MerC-AtVAM3 were found to localized in the Golgi apparatus, plasma membrane, and vacuole membrane, respectively. Transgenic Arabidopsis plants that expressed merC-SYP121 were more resistant to mercury and accumulated significantly more of this metal than wild-type Arabidopsis. These results demonstrated that expression of the bacterial heavy metal transporter MerC promoted the transport and accumulation of mercury in transgenic Arabidopsis, which may be a useful method for improving plants for the phytoremediation of mercury pollution. (C) 2012 Elsevier B.V. All rights reserved.