Production of Tiger Puffer Takifugu rubripes Offspring from Triploid Grass Puffer Takifugu niphobles Parents
Production of Tiger Puffer Takifugu rubripes Offspring from Triploid Grass Puffer Takifugu niphobles Parents
复制标题
DOI：
10.1007/s10126-017-9777-1
复制
发表时间：
2017-12-01
影响因子：
3
通讯作者：
Yoshizaki, Goro
中科院分区：
文献类型：
--
作者：
Hamasaki, Masaomi;Takeuchi, Yutaka;Yoshizaki, Goro
红鳍东方鲀是最受欢迎的水产养殖鱼类之一；然而，选择性育种有两个主要障碍。首先，它们的世代时间很长，需要2到3年才能成熟。其次，虎河豚亲代体型（2-5公斤）远大于市场平均体型（0.6-1.0公斤）。草河豚 Takifugu niphobles 与虎河豚关系密切，成熟时间缩短一半。此外，草河豚的成熟体重约为成年虎河豚的1/150，因此可以小面积饲养。因此，为了克服虎河鲀成熟体型和世代时间的障碍，我们通过生殖细胞移植培育出可以产生虎河鲀配子的代用草河鲀。孵化后1天，将大约5000个虎河豚睾丸细胞移植到三倍体草河豚幼虫的腹膜腔中。当受体鱼成熟时，雄性和雌性都会产生来自供体的配子。通过授精，我们成功地产生了供体虎河豚后代，其体表斑点图案、背鳍射线数量和DNA指纹与供体虎河豚相同，这表明受体草河豚产生了来自供体虎河豚的功能性卵子和精子。尽管仍需要进行微调以提高效率，但由于代草河豚的世代时间短且体型较小，预计将加速虎河豚的繁殖进程。
The tiger puffer Takifugu rubripes is one of the most popular aquacultural fish; however, there are two major obstacles to selective breeding. First, they have a long generation time of 2 or 3 years until maturation. Second, the parental tiger puffer has a body size (2-5 kg) much larger than average market size (0.6-1.0 kg). The grass puffer Takifugu niphobles is closely related to the tiger puffer and matures in half the time. Furthermore, grass puffer can be reared in small areas since their maturation weight is about 1/150 that of mature tiger puffer. Therefore, to overcome the obstacles of maturation size and generation time of tiger puffer, we generated surrogate grass puffer that can produce tiger puffer gametes through germ cell transplantation. Approximately 5000 tiger puffer testicular cells were transplanted into the peritoneal cavity of triploid grass puffer larvae at 1 day post hatching. When the recipient fish matured, both males and females produced donor-derived gametes. Through their insemination, we successfully produced donor-derived tiger puffer offspring presenting the same body surface dot pattern, number of dorsal fin rays, and DNA fingerprint as those of the donor tiger puffer, suggesting that the recipient grass puffer produced functional eggs and sperm derived from the donor tiger puffer. Although fine tunings are still needed to improve efficiencies, surrogate grass puffer are expected to accelerate the breeding process of tiger puffer because of their short generation time and small body size.