Antimicrobial activity of the volatile compound 3,5-dichloro-4-methoxybenzaldehyde, produced by the mushroom Porostereum spadiceum, against plant-pathogenic bacteria and fungi.
Antimicrobial activity of the volatile compound 3,5-dichloro-4-methoxybenzaldehyde, produced by the mushroom Porostereum spadiceum, against plant-pathogenic bacteria and fungi.
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由蘑菇 Poostereum spadiceum 产生的挥发性化合物 3,5-二氯-4-甲氧基苯甲醛对植物病原细菌和真菌具有抗菌活性。
DOI：
10.1111/jam.15020
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发表时间：
2021
影响因子：
4
通讯作者：
H.Otani and K.Osaki-Oka
中科院分区：
文献类型：
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作者：
E.Hamamoto;N.Kimura;S.Nishino;A.Ishihara;H.Otani and K.Osaki-Oka
目的研究香菇菌丝体释放的挥发性化合物对植物病原菌和真菌的抑制活性。方法与结果筛选结果表明，香菇菌丝体挥发性化合物对植物病原菌的菌落形成有明显的抑制作用，对植物病原菌的分生孢子萌发也有明显的抑制作用。从培养滤液OFP中分离得到挥发性化合物。Spadiceum和3，4-二氯-4-甲氧基苯甲醛(DCMB)为主要成分。10μg m l−1对细菌定植有明显抑制作用，0.1-1μg m l−1水蒸气对真菌分生孢子萌发有明显抑制作用。研究了DCMB在低浓度下对植物病原菌和真菌的抑菌活性。这项研究的意义和影响为了解蘑菇挥发性化合物的抗菌活性提供了了解，该化合物可能作为一种新型的生防剂用于保护各种植物病害。
AimsIn this study, volatile compounds released from mycelia of some aromatic mushrooms were investigated for their inhibitory activity against plant‐pathogenic bacteria and fungi.Methods and ResultsA screening revealed that volatile compounds from mycelia ofPorostereum spadiceumremarkably inhibited the colony formation of plant‐pathogenic bacteria, includingClavibacter michiganensissubsp.michiganensisandRalstonia solanacearumwhile also inhibiting the conidial germination of plant‐pathogenic fungi includingAlternaria brassicicolaandColletotrichum orbiculare. The volatile compounds were isolated from the culture filtrate ofP. spadiceum, and 3,4‐dichloro‐4‐methoxybenzaldehyde (DCMB) was identified as a major compound. DCMB significantly inhibited bacterial colonization at 10μg ml−1and fungal conidial germination at 0·1–1μg ml−1as a vapour.ConclusionsThis is the first report on the production of the volatile compound DCMB byP. spadiceumand on the antimicrobial activity of DCMB against plant‐pathogenic bacteria and fungi at low concentrations. It may be possible to use the compound as an agent for protecting crops from bacterial and fungal diseases during cultivation and storage.Significance and Impact of the StudyThis study provides an understanding of antimicrobial activity of the mushroom volatile compound that may be useful as a novel biological control agent for protecting various plant diseases.