Surface functionalized mesoporous silica nanoparticles with natural proteins for reduced immunotoxicity
Surface functionalized mesoporous silica nanoparticles with natural proteins for reduced immunotoxicity
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具有天然蛋白质的表面功能化介孔二氧化硅纳米颗粒可降低免疫毒性
DOI：
10.1002/jbm.a.35049
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发表时间：
2014-11-01
影响因子：
4.9
通讯作者：
Cai, Kaiyong
中科院分区：
文献类型：
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作者：
Luo, Zhong;Hu, Yan;Cai, Kaiyong
介孔二氧化硅纳米颗粒（MSN）是最有前途的药物输送纳米载体之一。为了降低其免疫毒性，在本研究中，通过使用琥珀酸酐作为中间连接体，使用明胶（Gel）、牛血清白蛋白（BSA）和溶菌酶（Lys）的天然蛋白质作为MSNs的端帽，从而分别制备MSNs/蛋白质纳米复合材料。此外，还利用SEM、TEM、FTIR和zeta电位仪器的组合技术分别监测MSNs/蛋白质纳米复合材料的构建过程。最后，详细研究了这些纳米复合材料对巨噬细胞（RAW264.7细胞）的免疫毒性，即细胞形态、细胞活力、一氧化氮（NO）产生、活性氧（ROS）和酸性磷酸酶活性（ACP）以及炎症细胞因子表达（肿瘤坏死因子-α和白介素-1β）。所有结果表明，巨噬细胞在摄取 SiO2 和 MSN 纳米颗粒后被激活，随后在体外诱导严重的炎症反应。相比之下，MSNs 纳米复合材料的炎症反应在用这些天然蛋白质封端后显着降低。总体而言，这项研究为开发基于 MSN 的免疫毒性降低的药物递送系统积累了知识。 (C) 2013 年 Wiley 期刊公司。
Mesoporous silica nanoparticles (MSNs) present themselves as one of the most promising nano-carriers for drug delivery. To reduce their immunotoxicities, in this study, natural proteins of gelatin (Gel), bovine serum albumin (BSA), and lysozyme (Lys) were employed as end-caps of MSNs by using succinic anhydride as an intermediate linker, thus leading to fabrication of MSNs/protein nanocomposites, respectively. Furthermore, combined techniques of SEM, TEM, FTIR, and zeta potential instruments were utilized to monitor the construction processes of MSNs/protein nanocomposites, respectively. Finally, the immunotoxicities of those nanocomposites to macrophage cells (RAW264.7 cells) were investigated in detail, i.e., cell morphology, cell viability, nitric oxide (NO) production, reactive oxygen species (ROS), and acid phosphatase activity (ACP) as well as inflammation cytokine expressions (tumor necrosis factor-alpha and interleukin-1 beta). All results suggest that macrophages were activated after uptaking nanoparticles of SiO2 and MSNs, which subsequently induced severe inflammation responses in vitro. In contrast, the inflammation responses of MSNs nanocomposites were reduced dramatically after end-capping with those natural proteins. Overall, this study accumulates knowledge for the development of MSNs-based drug delivery systems with reduced immunotoxicity. (C) 2013 Wiley Periodicals, Inc.