Microbial degradation of polycyclic aromatic hydrocarbons in soil by bacterium-fungus co-cultures
Microbial degradation of polycyclic aromatic hydrocarbons in soil by bacterium-fungus co-cultures
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DOI：
10.1007/bf02931064
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发表时间：
2007-07-01
影响因子：
3.2
通讯作者：
Lee, Choul-Gyun
中科院分区：
文献类型：
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作者：
Kim, Jeong-Dong;Lee, Choul-Gyun
以两种真菌和降解菲的红球菌（Rhodococcus sp. IC10）为接种剂，对某制气厂区石油烃污染土壤进行了生物修复。这两种真菌先前从不同的烃污染土壤样品中分离得到，鉴定为土曲霉和青霉。此外，采用两种类型的真菌，包括A. terreus或青霉菌与红球菌sp. IC10共培养。经过10周的孵育期，16种多环芳烃（PAH）的蒽、菲和芘的浓度在第68、54和64天被完全生物降解。生物修复后土壤的生态毒性对大水蚤（24 h龄水蚤）的存活没有影响。但对白芥种子萌发的毒性和蜡样芽孢杆菌的氧化还原酶活性分别在5周和10周后下降。青霉菌与红球菌IC10共培养降低生态毒性效果最好。(c) KSBB。
Two fungi and the phenanthrene-degrading bacterial strain Rhodococcus sp. IC10 were used as inocula for the bioremediation of petroleum hydrocarbon-contaminated soil from a manufactured gas plant area. The two fungi, which were previously isolated from different hydrocarbon-contaminated soil samples, were identified as Aspergillus terreus and Penicillium sp. In addition, two types of co-cultures which consist of fungal species including A. terreus or Penicillium sp. with Rhodococcus sp. IC10 were applied. After a 10-week incubation period, the concentrations of anthracene, phenanthrene, and pyrene were totally biodegraded by days 68, 54, and 64, for the 16 polycyclic aromatic hydrocarbons (PAH's) tested. The ecotoxicity of the soil after bioremediation did not show any effect on the survival of Daphnia magna (24 h-old-daphnids). However, the toxicity on seed germination of Brassica alba and the oxidoreductase activity of Bacillus cereus declined after 5- and 10-weeks of incubation, respectively. Co-cultures of Penicillium sp. and Rhodococcus sp. IC10 revealed the best efficiency at reducing ecotoxicity. (c) KSBB.