Stabilization of chromatin structure by PRC1, a polycomb complex
Stabilization of chromatin structure by PRC1, a polycomb complex
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DOI：
10.1016/s0092-8674(00)80604-2
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发表时间：
1999-07-09
期刊：
影响因子：
64.5
通讯作者：
Kingston, RE
中科院分区：
文献类型：
--
作者：
Shao, ZH;Raible, F;Kingston, RE
多梳群（Polycomb group, PcG）基因是在发育过程中维持同源基因抑制所必需的。这些基因的突变可以被SWI/SNF家族基因的突变所抑制。我们纯化了一种复合物，称为PRC1 (Polycomb suppressuppresscomplex 1)，它包含PcG基因Polycomb、Posterior sex comb、polyhomeotic、sex comb on midleg和其他几种蛋白质的产物。PRC1与核小体阵列的预孵育阻断了这些阵列被SWI/SNF重塑的能力。将PRC1与SWI/SNF同时加入到阵列中并不会阻断重构。因此，PRC1和SWI/SNF可能相互竞争核小体模板。几种不同类型的抑制复合物，包括去乙酰化酶，与组蛋白尾部相互作用。相反，PRC1在无尾组蛋白形成的核小体阵列上有活性。
The Polycomb group (PcG) genes are required for maintenance of homeotic gene repression during development. Mutations in these genes can be suppressed by mutations in genes of the SWI/SNF family. We have purified a complex, termed PRC1 (Polycomb repressive complex 1), that contains the products of the PcG genes Polycomb, Posterior sex combs, polyhomeotic, Sex combs on midleg, and several other proteins. Preincubation of PRC1 with nucleosomal arrays blocked the ability of these arrays to be remodeled by SWI/SNF. Addition of PRC1 to arrays at the same time as SWI/SNF did not block remodeling. Thus, PRC1 and SWI/SNF might compete with each other for the nucleosomal template. Several different types of repressive complexes, including deacetylases, interact with histone tails. In contrast, PRC1 was active on nucleosomal arrays formed with tailless histones.