Effects of hyperbaric oxygen on MMP-2 and MMP-9 expression and spinal cord edema after spinal cord injury.
Effects of hyperbaric oxygen on MMP-2 and MMP-9 expression and spinal cord edema after spinal cord injury.
复制标题
高压氧对脊髓损伤后MMP-2、MMP-9表达及脊髓水肿的影响
作者：
Jing Yang;Guozhong Wang;Chun;Gui;N. Kang
目标管理系统
评价高压氧（HBO）治疗对急性脊髓损伤（SCI）后MMP-2和MMP-9表达及脊髓水肿的影响。
主要方法
96 只健康成年 Sprague – Dawley 大鼠被随机分为 4 组：假手术组、假手术 + HBO、SCI 和 SCI + HBO。每组又分为4个亚组，每组6只大鼠：损伤后24小时、48小时、72小时和5天。使用 ELISA 测定基质金属蛋白酶 2 (MMP-2)、MMP-9、白细胞介素 (IL-6) 和血管内皮生长因子 (VEGF) 的表达水平。还通过艾略特方程测量了脊髓的水含量。
关键发现
SCI后不同时间点与假手术组和假手术+HBO组相比，SCI组MMP-2和MMP-9水平以及脊髓含水量均显着升高（P < 0.01）。 SCI组与SCI+HBO组在SCI后72 h时MMP-2水平差异有统计学意义（P < 0.05），而MMP-9水平在SCI后48 h、72 h、5 d时差异有统计学意义（P < 0.05）。 SCI 后 5 d 时 SCI 组脊髓含水量显着升高（P < 0. 01）。 MMP-2（P < 0.05）和MMP-9（P < 0.01）水平与脊髓含水量呈正相关。
意义
HBO通过下调IL-6、MMP-2、MMP-9的表达，上调VEGF的表达，减轻SCI引起的脊髓水肿，稳定血脊髓屏障，促进神经元功能恢复。
AIMS
To evaluate the effects of hyperbaric oxygen (HBO) therapy on MMP-2 and MMP-9 expression and spinal cord edema after acute spinal cord injury (SCI).
MAIN METHODS
Ninety-six healthy adult Sprague − Dawley rats were randomly divided into 4 groups: sham, sham + HBO, SCI, and SCI + HBO. Each group was subdivided into 4 subgroups of 6 rats each: 24 h, 48 h, 72 h, and 5 d post-injury. The expression levels of matrix metalloproteinase-2 (MMP-2), MMP-9, interleukin (IL-6), and vascular endothelial growth factor (VEGF) were measured using ELISA. The water content of the spinal cord was also measured by Elliot equation.
KEY FINDING
MMP-2 and MMP-9 levels and spinal cord water content increased significantly in the SCI group compared to those in the sham and sham + HBO groups at different time points after SCI (P < 0.01). The MMP-2 level was significantly different between the SCI and SCI + HBO groups at 72 h after SCI (P < 0.05), while the MMP-9 level was significantly different at 48 h, 72 h, and 5 d (P < 0.05). The water content of the spinal cord was significantly higher in the SCI group at 5 d after SCI (P < 0. 01). MMP-2 (P < 0.05) and MMP-9 (P < 0.01) levels were positively correlated with spinal cord water content.
SIGNIFICANCE
HBO reduced SCI-induced spinal cord edema, stabilized the blood-spinal cord barrier, and promoted recovery of neuronal function by down regulating the expression of IL-6, MMP-2, and MMP-9 and up regulating the expression of VEGF.