High density DNA data storage library via dehydration with digital microfluidic retrieval
High density DNA data storage library via dehydration with digital microfluidic retrieval
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DOI：
10.1038/s41467-019-09517-y
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发表时间：
2019-04-12
影响因子：
16.6
通讯作者：
Ceze, Luis
中科院分区：
文献类型：
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作者：
Newman, Sharon;Stephenson, Ashley P.;Ceze, Luis
DNA有望成为高密度数据存储介质，但物理存储提出了挑战。要存储大量数据，池必须在物理上隔离，以便它们可以共享相同的寻址方案。我们提出了一种可扩展的DNA数据存储的方法，在玻璃上的脱水DNA点的存储。然后可以使用数字微流体装置通过水滴取回干燥的斑点。在这里，我们表明，这种存储模式与不同的现场组织，斑点质量的DNA，和液滴检索停留时间。在所有情况下，大部分DNA都被检索并成功测序。我们证明了斑点可以密集地排列在微流体装置上，而不会对检索造成显著污染。我们还证明了1 TB的数据可以存储在一个DNA点中，并使用这种方法成功检索。
DNA promises to be a high density data storage medium, but physical storage poses a challenge. To store large amounts of data, pools must be physically isolated so they can share the same addressing scheme. We propose the storage of dehydrated DNA spots on glass as an approach for scalable DNA data storage. The dried spots can then be retrieved by a water droplet using a digital microfluidic device. Here we show that this storage schema works with varying spot organization, spotted masses of DNA, and droplet retrieval dwell times. In all cases, the majority of the DNA was retrieved and successfully sequenced. We demonstrate that the spots can be densely arranged on a microfluidic device without significant contamination of the retrieval. We also demonstrate that 1 TB of data could be stored in a single spot of DNA and successfully retrieved using this method.