Serological and molecular prevalence of Borrelia burgdorferi, Anaplasma phagocytophilum, and Ehrlichia species in dogs from Minnesota
Serological and molecular prevalence of Borrelia burgdorferi, Anaplasma phagocytophilum, and Ehrlichia species in dogs from Minnesota
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DOI：
10.1089/vbz.2007.0236
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发表时间：
2008-08-01
影响因子：
2.1
通讯作者：
Breitschwerdt, Edward B.
中科院分区：
文献类型：
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作者：
Beall, Melissa J.;Chandrashekar, Ramaswamy;Breitschwerdt, Edward B.
明尼苏达州巴克斯特（莱姆病和无形体病流行地区）的一家私人诊所对731只自然接触的宠物狗进行了检查，通过血清学和分子方法进行了检测，以寻找接触或感染选定媒介传播病原体的证据。通过酶联免疫吸附试验（ELISA）检测血清样本中的嗜吞噬细胞无形体、伯氏疏螺旋体和犬埃立克体抗体以及犬恶丝虫抗原。还通过聚合酶链反应（PCR）分析了273只犬的血液样本中的无形体属和埃立克体属DNA。根据主人病史和主治兽医的体格检查结果，将表现出与无形体病或疏螺旋体病相容的疾病的犬视为临床病例，并将其结果与健康犬群进行比较。抗体仅针对A.在217只（29%）犬中检测到嗜吞噬细胞菌;仅B。在80只（11%）犬中检测到伯氏螺旋体;在188只（25%）犬中检测到对两种微生物的血清反应性。在89例疑似犬无形体病或疏螺旋体病的病例中，A.嗜吞噬细胞菌或B。分别在22只狗（25%）及8只狗（9%）中检测到伯氏抗体，而在38只狗（43%）中同时检测到两种微生物的抗体。犬埃立克体抗体和D.在11只（1.5%）犬中分别检测到了免疫抗原。从222只健康犬中的7只（3%）和51例临床病例中的19只（37%）中扩增出嗜吞噬细胞无形体DNA。血清反应性均为A.嗜吞噬细胞菌和B.在疑似无形体病和/或疏螺旋体病病例中检测到伯氏螺旋体的频率高于单独对任一生物体的血清反应性。通过PCR检测，A.在疑似无形体病或疏螺旋体病的病例中，嗜吞噬细胞DNA比来自同一地区的健康犬更普遍。
A population of 731 naturally exposed pet dogs examined at a private practice in Baxter, Minnesota, an area endemic for Lyme disease and anaplasmosis, was tested by serological and molecular methods for evidence of exposure to or infection with selected vector-borne pathogens. Serum samples were tested by enzyme-linked immunosorbent assay (ELISA) for Aanaplasma phagocytophilum, Borrelia burgdorferi, and Ehrlichia canis antibodies and for Dirofilaria immitis antigen. Blood samples from 273 dogs were also analyzed by polymerase chain reaction (PCR) for Anaplasma and Ehrlichia species DNA. Based on the owner history and the attending veterinarian's physical examination findings, dogs exhibiting illness compatible with anaplasmosis or borreliosis were considered clinical cases, and their results were compared to the healthy dog population. Antibodies to only A. phagocytophilum were detected in 217 (29%) dogs; to only B. burgdorferi, in 80 (11%) dogs; and seroreactivity to both organisms, in 188 (25%) dogs. Of 89 suspected cases of canine anaplasmosis or borreliosis, A. phagocytophilum or B. burgdorferi antibodies were detected in 22 dogs (25%) and 8 dogs (9%) respectively, whereas antibodies to both organisms were found in 38 dogs (43%). Ehrlichia canis antibodies and D. immitis antigen were each detected in 11 (1.5%) dogs. Anaplasma phagocytophilum DNA was amplified from 7 of 222 (3%) healthy dogs and 19 of 51 (37%) clinical cases. Seroreactivity to both A. phagocytophilum and B. burgdorferi was detected more frequently in suspected cases of anaplasmosis and/or borreliosis than seroreactivity to either organism alone. Based on PCR testing, A. phagocytophilum DNA was more prevalent in suspected cases of anaplasmosis or borreliosis than in healthy dogs from the same region.