Glucosinolate profiles in broccoli: Variation in levels and implications in breeding for cancer chemoprotection
Glucosinolate profiles in broccoli: Variation in levels and implications in breeding for cancer chemoprotection
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DOI：
10.21273/jashs.127.5.807
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发表时间：
2002-09-01
影响因子：
1.9
通讯作者：
Juvik, JA
中科院分区：
文献类型：
--
作者：
Brown, AF;Yousef, GG;Juvik, JA
将10份西兰花[甘蓝（Brassica oleracea L.）（花椰菜组）]材料种植在多种环境中，以评估与基因型、环境以及基因型×环境互作相关的硫代葡萄糖苷（GS）变异性，并确定西兰花小花中硫代葡萄糖苷稳定性的差异。在脂肪族硫代葡萄糖苷中发现了显著的遗传变异性差异，但在吲哚族硫代葡萄糖苷中未发现。单个脂肪族化合物中可归因于基因型的硫代葡萄糖苷变异性百分比，从萝卜硫苷的54.2%到致甲状腺肿素的71.0%不等。对于总吲哚族硫代葡萄糖苷，可归因于基因型的变异性百分比仅为12%。在基因型之间检测到硫代葡萄糖苷的定性和定量差异。在最高和最低基因型之间，观察到致甲状腺肿素、萝卜硫苷和总脂肪族硫代葡萄糖苷水平存在10倍的差异。只有两个品系，Eu8 - 1和VI - 158，产生了大量除萝卜硫苷以外的脂肪族硫代葡萄糖苷。在秋季和春季种植之间，还观察到这些化合物在品种间稳定性的差异。结果表明，改变脂肪族硫代葡萄糖苷定性和定量特征所需的遗传因素存在于现有的西兰花种质资源中，这使得培育具有增强的癌症化学预防活性的品种具有可行性。
Ten broccoli [Brassicaoleracea L. (Botrytis Group)] accessions were grown in several environments to estimate glucosinolate (GS) variability associated with genotype, environment, and genotype x environment interaction and to identify differences in the stability of GSs in broccoli florets. Significant differences in genetic variability were identified for aliphatic GSs but not for indolyl GSs. The percentage of GS variability attributable to genotype for individual aliphatic compounds ranged from 54.2 % for glucoraphanin to 71.0% for progoitrin. For total indolyl GSs, the percentage of variability attributable to genotype was only 12 %. Both qualitative and quantitative differences in GSs were detected among the genotypes. Ten-fold differences in progoitrin, glucoraphanin, and total aliphatic GS levels were observed between the highest and lowest genotypes. Only two lines, Eu8-1 and VI-158, produced aliphatic GSs other than glucoraphanin in appreciable amounts. Differences in stability of these compounds among the cultivars were also observed between fall and spring plantings. Results suggest that genetic factors necessary for altering the qualitative and quantitative aliphatic GS profiles are present within existing broccoli germplasm, which makes breeding for enhanced cancer chemoprotectant activity feasible.