Making the Leap from Research Laboratory to Clinic: Challenges and Opportunities for Next-Generation Sequencing in Infectious Disease Diagnostics.
Making the Leap from Research Laboratory to Clinic: Challenges and Opportunities for Next-Generation Sequencing in Infectious Disease Diagnostics.
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DOI：
10.1128/mbio.01888-15
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发表时间：
2015-12-08
期刊：
影响因子：
6.4
通讯作者：
Weinstock GM
中科院分区：
文献类型：
--
作者：
Goldberg B;Sichtig H;Geyer C;Ledeboer N;Weinstock GM
下一代DNA测序（NGS）在过去十年中取得了巨大进展，改变了基因组分析，并为临床微生物学实验室的应用开辟了许多新的机会。NGS对微生物学的影响是革命性的，每天都有新的微生物基因组序列产生，导致大型基因组和基因序列数据库的发展。在不培养生物体的情况下分析微生物群落的能力创造了不断增长的宏基因组学和微生物组分析领域，并对宿主和微生物之间的关系产生了重要的新见解。医学文献中有许多关于这种新技术如何用于传染病诊断和病原体分析的例子。NGS在医疗实践中的实施一直是一个缓慢的过程，由于各种挑战，如临床试验，缺乏适用的监管指南，以及该技术对临床环境的适应。2015年4月，美国微生物学会（AAM）召开了一次座谈会，开始定义这些问题，在本文中，我们介绍了从这些讨论中产生的一些概念。
Next-generation DNA sequencing (NGS) has progressed enormously over the past decade, transforming genomic analysis and opening up many new opportunities for applications in clinical microbiology laboratories. The impact of NGS on microbiology has been revolutionary, with new microbial genomic sequences being generated daily, leading to the development of large databases of genomes and gene sequences. The ability to analyze microbial communities without culturing organisms has created the ever-growing field of metagenomics and microbiome analysis and has generated significant new insights into the relation between host and microbe. The medical literature contains many examples of how this new technology can be used for infectious disease diagnostics and pathogen analysis. The implementation of NGS in medical practice has been a slow process due to various challenges such as clinical trials, lack of applicable regulatory guidelines, and the adaptation of the technology to the clinical environment. In April 2015, the American Academy of Microbiology (AAM) convened a colloquium to begin to define these issues, and in this document, we present some of the concepts that were generated from these discussions.