Combined sugar yields for dilute sulfuric acid pretreatment of corn stover followed by enzymatic hydrolysis of the remaining solids
Combined sugar yields for dilute sulfuric acid pretreatment of corn stover followed by enzymatic hydrolysis of the remaining solids
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DOI：
10.1016/j.biortech.2005.01.011
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发表时间：
2005-12-01
影响因子：
11.4
通讯作者：
Wyman, CE
中科院分区：
文献类型：
--
作者：
Lloyd, TA;Wyman, CE
许多先前的研究确定了稀酸预处理条件，以最大限度地提高预处理木糖的产量或预处理后纤维素的后续消化葡萄糖的产量，但我们的重点是确定在两个阶段实现两种糖的最高产量的条件。因此,个人木糖和葡萄糖产量报告的潜在产量的比例糖在一系列的硫酸浓度为0.22%,0.49%和0.98% w / w在140年,160年,180年和200度c高达15%的潜在糖底物可以被释放葡萄糖在预处理和15%至90 + %木糖剩余的固体残渣可以恢复在随后的酶法水解,根据酶加载。葡萄糖产率从仅酶解时总最大电位葡萄糖加木糖的56%增加到预处理时释放的葡萄糖的60%。同样，木糖的产量从单独预处理的高达总潜在糖的34%增加到消化释放木糖的35%至37%。如果不使用酸，产量会低得多。使单个糖产量最大化的条件往往与使总糖产量最大化的条件不同，这表明在优化过程时明确定义预处理目标的重要性。总体而言，通过稀酸预处理和酶解耦合，可回收玉米秸秆中原可用总糖的92.5%。这些结果还表明，增强半纤维素酶活性可以进一步提高木糖产量，特别是在低纤维素酶负荷下。(c) 2005 Elsevier Ltd版权所有。
A number of previous studies determined dilute acid pretreatment conditions that maximize xylose yields from pretreatment or glucose yields from subsequent digestion of the pretreated cellulose, but our emphasis was on identifying conditions to realize the highest yields of both sugars from both stages. Thus, individual xylose and glucose yields are reported as a percentage of the total potential yield of both sugars over a range of sulfuric acid concentrations of 0.22%, 0.49% and 0.98% w/w at 140, 160, 180 and 200 degrees C. Up to 15% of the total potential sugar in the substrate could be released as glucose during pretreatment and between 15% and 90+% of the xylose remaining in the solid residue could be recovered in subsequent enzymatic hydrolysis, depending on the enzyme loading. Glucose yields increased from as high as 56% of total maximum potential glucose plus xylose for just enzymatic digestion to 60% when glucose released in pretreatment was included. Xylose yields similarly increased from as high as 34% of total potential sugars for pretreatment alone to between 35% and 37% when credit was taken for xylose released in digestion. Yields were shown to be much lower if no acid was used. Conditions that maximized individual sugar yields were often not the same as those that maximized total sugar yields, demonstrating the importance of clearly defining pretreatment goals when optimizing the process. Overall, up to about 92.5% of the total sugars originally available in the corn stover used could be recovered for coupled dilute acid pretreatment and enzymatic hydrolysis. These results also suggest that enhanced hemicellulase activity could further improve xylose yields, particularly for low cellulase loadings. (c) 2005 Elsevier Ltd. All rights reserved.