Adsorption-Elution Techniques and Molecular Detection of Enteric Viruses from Water
Adsorption-Elution Techniques and Molecular Detection of Enteric Viruses from Water
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水中肠道病毒的吸附洗脱技术和分子检测
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
J. Gichana
中科院分区：
文献类型：
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作者：
Vurayai Ruhanya;ry Kabego;J. Gichana
市政当局和公共卫生当局普遍认为，通过计数粪便大肠菌群来监测微生物水质。使用这些粪便污染的细菌指标是因为假设它们在水中与肠道病原体具有相同的行为，但对于病毒而言，情况并非如此。因此，需要补充病毒指示剂或直接检测水中的病毒。由于病毒的数量很少，因此水中病毒的检测很复杂。需要将病毒从大量水浓缩成可用于 PCR 或细胞培养的等分试样。我们提出了用于从水中回收和浓缩肠道病毒以及随后通过定量实时 RT-PCR/PCR 进行检测的吸附洗脱技术。
It is generally accepted by municipalities and public health authorities that monitoring microbial water quality is performed by enumerating faecal coliforms. These bacterial indicators of faecal contamination are used because of the assumption that they have the same behaviour in the water as enteric pathogens, which is not true with regards to viruses. Therefore, there is need for a supplementary viral indicator or to directly detect viruses from water. Detection of viruses in water is complicated because of occurrence of viruses in low numbers. There is need for concentration of the viruses from large volumes water to aliquots that can be used in PCR or cell culture. We present the adsorption-elution techniques for the recovery and concentration of enteric viruses from water and subsequent detection by quantitative real time RT-PCR/PCR.