DOMINO1, a member of a small plant-specific gene family, encodes a protein essential for nuclear and nucleolar functions
DOMINO1, a member of a small plant-specific gene family, encodes a protein essential for nuclear and nucleolar functions
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DOI：
10.1111/j.1365-313x.2004.02166.x
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发表时间：
2004-09-01
期刊：
影响因子：
7.2
通讯作者：
Devic, M
中科院分区：
文献类型：
--
作者：
Lahmy, S;Guilleminot, J;Devic, M
与野生型胚胎相比，携带domino1突变的拟南芥胚胎生长缓慢，因此在干燥时仅达到球形阶段。细胞水平突变的主要缺陷是受精后不久即可在胚胎和胚乳的细胞核中观察到的大核仁。突变核仁的超微结构与野生型截然不同，表明核糖体生物合成存在缺陷。 DOMINO1 编码的蛋白质属于植物特异性基因家族，具有未知功能的共同基序，存在于番茄缺陷叶绿体和叶子 (LeDCL) 蛋白质中。使用 GFP 蛋白融合，我们证明 DOMINO1 靶向细胞核。我们认为 DOMINO1 失活会对核糖体生物发生和细胞分裂速率产生负面影响。
Arabidopsis embryos carrying the domino1 mutation grow slowly in comparison with wild type embryos and as a consequence reach only the globular stage at desiccation. The primary defect of the mutation at the cellular level is the large size of the nucleolus that can be observed soon after fertilization in the nuclei of both the embryo and the endosperm. The ultrastructure of mutant nucleoli is drastically different from wild type and points to a fault in ribosome biogenesis. DOMINO1 encodes a protein, which belongs to a plant-specific gene family sharing a common motif of unknown function, present in the tomato DEFECTIVE CHLOROPLASTS AND LEAVES (LeDCL) protein. Using a GFP protein fusion, we show that DOMINO1 is targeted to the nucleus. We propose that inactivation of DOMINO1 has a negative effect on ribosome biogenesis and on the rate of cell division.