Three-dimensional nanofibrillar surfaces covalently modified with tenascin-C-derived peptides enhance neuronal growth in vitro
Three-dimensional nanofibrillar surfaces covalently modified with tenascin-C-derived peptides enhance neuronal growth in vitro
复制标题
DOI：
10.1002/jbm.a.30587
复制
发表时间：
2006-03-15
影响因子：
4.9
通讯作者：
Meiners, S
中科院分区：
文献类型：
--
作者：
Ahmed, I;Liu, HY;Meiners, S
目前促进培养神经元生长的方法是使用二维(2D)玻璃或聚苯乙烯表面包覆带电分子(如多L赖氨酸)或分离的细胞外基质蛋白(如层粘连蛋白-1)。然而，这些2D表面代表了在体内调节神经元生长的组装的ECM的三维(3D)结构的糟糕的拓扑近似。在这里，我们报道了一种新的3D合成纳米纤维表面的开发，用于神经元的培养。这种纳米纤维表面由聚酰胺纳米纤维组成，其组织结构模仿了ECM的孔隙率和几何形状。在纳米纤维和PLL包被的玻璃片上，小脑颗粒、大脑皮质、海马区、运动神经节和背根神经节神经元的神经元黏附和轴突生长相似，但轴突生成增加，而且用来自人Teriascin-C的神经活性多肽共价修饰纳米纤维显著增强了纳米纤维在体外促进神经元附着、轴突生成和轴突延伸的能力。因此，3D纳米纤维表面为神经元提供了物理和化学稳定的细胞培养表面，并潜在地为开发多肽修饰基质提供了一个令人兴奋的新机会，用于设计促进中枢神经系统损伤后轴突再生的策略。(C)2005年威利期刊公司。
Current methods to promote growth Of Cultured neurons use two-dimensional (2D) glass or polystyrene Surfaces coated with a charged molecule (e.g. poly-L-lysine (PLL)) or an isolated extracellular matrix (ECM) protein (e.g. laminin-1.). However, these 2D Surfaces represent a poor topological approximation of the three-dimensional (3D) architecture of the assembled ECM that regulates neuronal growth ill vivo. Here we report on the development of a new 3D synthetic nanofibrillar surface for the culture of neurons. This nanofibrillar surface is composed of polyamide nanofibers whose organization mimics the porosity and geometry of the ECM. Neuronal adhesion and neurite outgrowth from cerebellar granule, cerebral cortical, hippocampal, motor, and dorsal root ganglion neurons were similar on nanofibers and PLL-coated glass coverslips; however, neurite generation was increased, Moreover, covalent modification of the nanofibers with neuroactive peptides derived from human teriascin-C significantly enhanced the ability of the nanofibers to facilitate neuronal attachment, neurite generation, and neurite extension in vitro. Hence the 3D nanofibrillar surface provides a physically and chemically stabile cell Culture surface for neurons and, potentially, an exciting new opportunity for the development of peptide-modified matrices for use in strategies designed to encourage axonal regrowth following central nervous system injury. (c) 2005 Wiley Periodicals, Inc.