Microfluidic device for the rapid coating of magnetic cells with polyelectrolytes
Microfluidic device for the rapid coating of magnetic cells with polyelectrolytes
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DOI：
10.1016/j.matlet.2012.12.084
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发表时间：
2013-03-01
影响因子：
3
通讯作者：
Pamme, Nicole
中科院分区：
文献类型：
--
作者：
Tarn, Mark D.;Fakhrullin, Rawil. F.;Pamme, Nicole
我们展示了一种在微流体室内连续流动的情况下，将一层聚合物涂覆到磁改性酵母细胞（所谓的“机器人细胞”）上的快速方法。在整个腔室中产生聚电解质和洗涤缓冲液的层流，磁性细胞通过外部磁铁依次偏转通过同流流，使聚电解质沉积到细胞上，然后立即进行洗涤步骤，所有这些都在不到 90 秒的时间内完成。这种简单的沉积技术有望实现此类机器人细胞的功能化，用于生物电子学、生物分析和毒性筛选等应用，同时添加更多的试剂流将能够制造多层胶囊。 (C) 2013 Elsevier B.V. 保留所有权利。
We demonstrate a rapid method of coating a layer of polymer onto magnetically modified yeast cells, so-called "cyborg cells", in continuous flow within a microfluidic chamber. Laminar flow streams of polyelectrolyte and washing buffers were generated across the chamber, and the magnetic cells were deflected sequentially through the co-flowing streams via an external magnet, allowing polyelectrolyte deposition onto the cells immediately followed by the washing step, all in less than 90 s. This simple deposition technique shows promise for the functionalization of such cyborg cells for applications including bioelectronics, bioanalysis, and toxicity screening, while the addition of more reagent streams would enable the fabrication of multilayered capsules. (C) 2013 Elsevier B.V. All rights reserved.