Glucosyltransferase phase variation in Streptococcus gordonii modifies adhesion to saliva-coated hydroxyapatite surfaces in a sucrose-independent manner.
Glucosyltransferase phase variation in Streptococcus gordonii modifies adhesion to saliva-coated hydroxyapatite surfaces in a sucrose-independent manner.
复制标题
戈登链球菌中的葡萄糖基转移酶相变以不依赖于蔗糖的方式改变对唾液涂覆的羟基磷灰石表面的粘附。
DOI：
10.1111/j.1399-302x.1992.tb00521.x
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发表时间：
1992
影响因子：
--
通讯作者：
Jones,GW
中科院分区：
文献类型：
--
作者：
Vickerman,MM;Clewell,DB;Jones,GW
在洗涤细胞粘附试验中，高（Spp+）和低（Spp−）水平的葡糖基转移酶（GTF）活性之间的戈登链球菌相变化导致Spp−菌株与唾液包被的羟基磷灰石（S-HA）的粘附性更高。特定的GTF突变体没有显示出这种反应。尽管在粘附试验条件下，洗涤过的Spp+细胞从蔗糖中产生的葡聚糖是Spp−细胞的5倍或更多，但蔗糖同样提高了两种表型对羟基磷灰石（HA）的粘附，但对S-HA的粘附没有影响。然而，这种效应不是蔗糖特异性的，因为等摩尔量的其他碳水化合物和NaCl将两种Spp类型的粘附力提高到与蔗糖相似的水平。粘附与细胞疏水性的相对变化无关。这些结果表明，除了GTF活性的变化，其他相关的变化粘附可能会发生在Spp相位变化。
Phase variation ofStreptococcos gordoniibetween high (Spp+) and low (Spp−) levels of glucosyltransferase (GTF) activity resulted in the greater adhesion of Spp−strains to saliva‐coated hydroxyapatite (S‐HA) in a washed‐cell adhesion test. Specific GTF mutants did not show this response. Although washed Spp+cells produced 5‐fold or more glucan from sucrose than Spp−cells did under the conditions of the adhesion test, sucrose elevated the adhesion of both phenotypes to hydroxyapatite (HA) equally, but had no effect on adhesion to S‐HA. This effect was not sucrose‐specific, however, because equimolar amounts of other carbohydrates and NaCl elevated adhesion of both Spp types to levels similar to those seen with sucrose. Adhesion did not correlate with relative changes in cell hydrophobicity. These results suggest that, in addition to changes in GTF activity, other changes relevant to adhesion may occur during Spp phase variation.