Release of Growth Factors from a Reinforced Collagen GAG Matrix Supplemented with Platelet Rich Plasma: Influence on Cultured Human Meniscal Cells
Release of Growth Factors from a Reinforced Collagen GAG Matrix Supplemented with Platelet Rich Plasma: Influence on Cultured Human Meniscal Cells
复制标题
DOI：
10.1002/jor.22495
复制
发表时间：
2014-02-01
影响因子：
2.8
通讯作者：
Rushton, Neil
中科院分区：
文献类型：
--
作者：
Howard, Daniel;Shepherd, Jenny H.;Rushton, Neil
半月板软骨损伤与加速关节磨损以及骨关节炎密切相关。通过专为半月板修复而设计的纤维增强胶原蛋白基质，从富含血小板的血浆（PRP）中输送生长因子，可以改善损伤。研究了 PRP 的组成、生长因子的释放以及对半月板细胞生长和基因表达的影响。 PRP 是使用 Harvest Smartprep (HS-PRP)、Cascade Fibrinet (CF-PRP) 和简单离心方案 (DC-PRP) 分别从四个供体中制备的。 CF-PRP 的血小板比例最高，其他血细胞类型很少。 HS-PRP 具有最高的血小板总数，但也含有高水平的红细胞和白细胞。吸收到胶原基质中，HS-PRP 释放最高水平的 TGF-1 和 PDGF-AB，DC-PRP 释放最多的 IGF-1。胶原基质的累积释放量为48ng/cm(3) IGF-1、96ng/cm(3) TGF-1和9.6ng/cm(3) PDGF-AB。含有 PRP 的胶原蛋白基质能够增加外周全血以上的半月板细胞数量，并上调聚集蛋白聚糖、I 型胶原蛋白 (1) 和弹性蛋白的基因表达（分别为 3.3 +/- 0.8 倍、2.9 +/- 0.6 倍、4.0 +/- 1.4 倍）。证明 PRP 与纤维增强胶原基质相结合可以影响半月板细胞，并可能用于治疗半月板缺陷。 (c) 2013 年骨科研究学会。由 Wiley periodicals, Inc. 出版。J Orthop Res 32:273-278，2014 年。
Damage to meniscal cartilage has been strongly linked to accelerated articular wear and consequently to osteoarthritis. Damage might be ameliorated by delivery of growth factors from platelet rich plasma (PRP) via a fiber reinforced collagen matrix designed for meniscal repair. PRP composition, release of growth factors, and influence on meniscal cell growth and gene expression were investigated. PRP was prepared using Harvest Smartprep (HS-PRP), Cascade Fibrinet (CF-PRP), and a simple centrifuge protocol (DC-PRP) from four donors each. CF-PRP had the highest ratio of platelets, with very few other blood cell types. HS-PRP had the highest total number of platelets but also contained high levels of red and white blood cells. Absorbed to collagen matrices HS-PRP released the highest levels of TGF-1 and PDGF-AB with DC-PRP the most IGF-1. Cumulative release from collagen matrix was 48ng/cm(3) IGF-1, 96ng/cm(3) TGF-1, and 9.6ng/cm(3) PDGF-AB. Collagen matrix with PRP was able to increase meniscal cell number above peripheral whole blood and up-regulated gene expression of Aggrecan, Collagen type I (1), and Elastin (3.3 +/- 0.8-fold, 2.9 +/- 0.6-fold, 4.0 +/- 1.4-fold, respectively). Demonstrating that PRP combined with fiber reinforced collagen matrix could influence meniscal cells and might be of use for treating meniscal defects. (c) 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:273-278, 2014.