Post-mitotic, differentiated myotubes efficiently produce retroviral vector from hybrid adeno-retrovirus templates
Post-mitotic, differentiated myotubes efficiently produce retroviral vector from hybrid adeno-retrovirus templates
复制标题
有丝分裂后分化的肌管从杂合腺逆转录病毒模板中有效产生逆转录病毒载体
作者：
Michael L. Roberts;T. Athanasopoulos;M. Pohlschmidt;G. Duisit;F. Cosset;George Dickson
我们已经检查了增殖的成肌细胞和有丝分裂后分化的肌管使用杂合腺-逆转录病毒载体作为模板产生逆转录病毒载体的能力。我们发现，腺病毒感染后 48 小时，成肌细胞产生的逆转录病毒载体达到峰值，浓度为 4.8 × 104 cfu/ml，并且在 96 小时时几乎检测不到。与成肌细胞相比，完全分化和部分分化的肌管都能产生持续增加的逆转录病毒载体水平，48小时达到峰值，分别为1.4 × 105 cfu/ml和1.8 × 105 cfu/ml。添加细胞周期抑制剂阿菲迪霉素 (5 μg/ml) 对完全分化的肌管的逆转录病毒载体的产生没有影响，但导致部分分化的肌管的载体产量增加 80%。因此表明逆转录病毒载体的产生在有丝分裂后肌管中更有效并且不依赖于肌肉细胞周期进展。
We have examined the ability of proliferating myoblasts and post-mitotic, differentiated myotubes to produce retroviral vector using hybrid adeno-retroviral vectors as templates. We show that production of retroviral vector from myoblasts peaks 48 h after adenoviral infection at 4.8 × 104 cfu/ml and is scarcely detectable by 96 h. Both fully and partially differentiated myotubes were able to generate a sustained increase in the levels of retroviral vector compared with myoblasts peaking 48 h at 1.4 × 105 cfu/ml and 1.8 × 105 cfu/ml, respectively. Addition of the cell cycle inhibitor aphidicolin (5 μg/ml) had no effect on the production of retroviral vector from fully differentiated myotubes, but resulted in an 80% increase in vector production from partially differentiated myotubes. Thus indicating that retroviral vector production is more efficient in post-mitotic myotubes and is independent of muscle cell cycle progression.
I 型遗传性酪氨酸血症小鼠模型的离体肝基因治疗。
影响因子：
4.2
作者：
Overturf,K;Al-Dhalimy,M;Manning,K;Ou,CN;Finegold,M;Grompe,M
通讯作者：
Grompe,M
包装细胞系特征和优化逆转录病毒载体滴度：国家基因载体实验室的经验。
DOI：
10.1089/104303400750001408
发表时间：
2000
期刊：
Human gene therapy.
影响因子：
--
作者：
Reeves,L;Smucker,P;Cornetta,K
通讯作者：
Cornetta,K