PYRUVATE PHOSPHATE DIKINASE - SEQUENCE OF THE HISTIDYL PEPTIDE, THE PYROPHOSPHORYL AND PHOSPHORYL CARRIER
PYRUVATE PHOSPHATE DIKINASE - SEQUENCE OF THE HISTIDYL PEPTIDE, THE PYROPHOSPHORYL AND PHOSPHORYL CARRIER
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DOI：
10.1021/bi00566a022
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发表时间：
1980-01-01
期刊：
影响因子：
2.9
通讯作者：
WOOD, HG
中科院分区：
文献类型：
--
作者：
GOSS, NH;EVANS, CT;WOOD, HG
共生拟杆菌丙酮酸磷酸二激酶含有一个关键的组氨酸残基，在该酶催化的反应中，该残基起到调节磷酸化部分转移的作用。含有这种必需组氨酸残基的胰蛋白酶多肽已经通过先前开发的两步程序分离出来。该多肽已被手工丹西尔-埃德曼程序测序，并被证明是NH2-Gly-Gly-Met-Thr-Ser-His-Ala-Ala-Val-Val-Ala-Arg-CO2H.该肽与以前从其他酶中分离的其他磷酸化组氨酸肽之间没有容易解释的同源性。据预测，该序列包含α。从蛋氨酸残基到羧基末端精氨酸残基的螺旋。
Bacteroides symbiosus pyruvate phosphate dikinase contains a pivotal histidyl residue which functions to mediate the transfer of phosphoryl moieties during the reaction catalyzed by the enzyme. The tryptic peptide which contains this essential histidyl residue has been isolted by a 2-step procedure developed previously. This peptide has been sequenced by the manual dansyl-Edman procedure and is shown to be NH2-Gly-Gly-Met-Thr-Ser-His-Ala-Ala-Val-Val-Ala-Arg-CO2H. There is no readily interpretable homology between this peptide and other phosphorylated histidyl peptides previously isolated from other enzymes. It is predicted that the sequence contains an .alpha. helix from the methionine residue through to the carboxyl terminal arginine residue.