Immunohistochemical study of p53 protein expression in Spitz nevus as compared with other melanocytic lesions
Immunohistochemical study of p53 protein expression in Spitz nevus as compared with other melanocytic lesions
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DOI：
10.1097/00000372-199512000-00003
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发表时间：
1995-12-01
影响因子：
1.1
通讯作者：
Lichtig, C
中科院分区：
文献类型：
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作者：
Bergman, R;Shemer, A;Lichtig, C
应用单克隆抗体BP 53 -12对26例Spitz痣（SN）、26例原发性皮肤恶性黑色素瘤（MM）、20例转移瘤和17例普通复合痣（CN）石蜡包埋切片进行免疫组化研究。在7例（35%）转移性乳腺癌的部分肿瘤细胞中检测到阳性反应，所有肿瘤细胞均显示出强核染色;在8例（31%）原发性乳腺癌中检测到阳性反应，其中7例显示出强核染色;在2例（7%）SN中检测到阳性反应，其中仅1例显示出强核染色;在所有氯化萘中均未检测到阳性反应。MM组和转移性MM组中一般阳性染色病变的频率，特别是强阳性染色病变的频率均在统计学上显著高于SN组和CN组中相应的频率。我们认为，使用单克隆抗体如BP 53 -12在石蜡切片上进行p53蛋白的免疫组化检测，特别是当显示出强核反应性时，可能被证明是MM与SN的组织病理学鉴别的一个有力工具。
The accumulation of p53 protein was studied immunohistochemically on paraffin-embedded sections of 26 Spitz nevi (SNs), 26 primary invasive cutaneous malignant melanomas (MMs), 20 metastases of MM, and 17 ordinary compound nevi (CNs), using monoclonal antibody BP53-12. Positive reactivity was detected in some of the tumor cells in seven (35%) metastatic MMs, all exhibiting strong nuclear staining; eight (31%) primary MMs, of which seven showed strong nuclear staining; two (7%) SNs, of which only one showed strong nuclear staining; and none of the CNs. The frequencies of the positively stained lesions in general, and the strongly positively stained lesions in particular, in the MM and metastatic MM groups were each statistically significantly higher than the respective frequencies in the SN and CN groups. We believe that the immunohistochemical detection of p53 protein with the use of monoclonal antibodies such as BP53-12 on paraffin sections, especially when strong nuclear reactivity is demonstrated, may prove to be an adjunctive tool in the histopathologic differentiation of MM from SN.