Diffraction tomography with Fourier ptychography.
Diffraction tomography with Fourier ptychography.
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DOI：
10.1364/optica.3.000827
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发表时间：
2016-08
期刊：
影响因子：
10.4
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Horstmeyer R;Chung J;Ou X;Zheng G;Yang C
本文提出了一种技术，图像的复杂折射率的样品在三维空间。唯一需要的硬件是一个标准的显微镜和一个LED阵列。该方法，称为傅立叶重叠关联层析成像（FPT），首先捕获一个序列的强度只有一个样本的角度变化的照明下的图像。然后，使用重叠关联和衍射层析成像的原理，计算解决了样品的三维结构。实验显微镜在Nyquist-Shannon采样极限（Sparrow极限分别为0.54和5.0 μm）下，在110 μm的总成像深度上，横向空间分辨率为0.39 μm，轴向分辨率为3.7 μm。与竞争方法不同，该技术定量测量主要透明和连续样品特征的体积折射率，而不需要干涉测量或任何移动部件。厚生物标本的宽视场重建表明在病理学和发育生物学中的潜在应用。
This paper presents a technique to image the complex index of refraction of a sample across three dimensions. The only required hardware is a standard microscope and an array of LEDs. The method, termed Fourier ptychographic tomography (FPT), first captures a sequence of intensity-only images of a sample under angularly varying illumination. Then, using principles from ptychography and diffraction tomography, it computationally solves for the sample structure in three dimensions. The experimental microscope demonstrates a lateral spatial resolution of 0.39 μm and an axial resolution of 3.7 μm at the Nyquist–Shannon sampling limit (0.54 and 5.0 μm at the Sparrow limit, respectively) across a total imaging depth of 110 μm. Unlike competing methods, this technique quantitatively measures the volumetric refractive index of primarily transparent and contiguous sample features without the need for interferometry or any moving parts. Wide field-of-view reconstructions of thick biological specimens suggest potential applications in pathology and developmental biology.