Transcriptome Analyses Reveal Candidate Pod Shattering-Associated Genes Involved in the Pod Ventral Sutures of Common Vetch (Vicia sativa L.).
Transcriptome Analyses Reveal Candidate Pod Shattering-Associated Genes Involved in the Pod Ventral Sutures of Common Vetch (Vicia sativa L.).
复制标题
转录组分析揭示了与野豌豆 (Vicia sativa L.) 荚果腹缝相关的候选荚果破碎相关基因
DOI：
10.3389/fpls.2017.00649
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发表时间：
2017
影响因子：
5.6
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Dong R;Dong D;Luo D;Zhou Q;Chai X;Zhang J;Xie W;Liu W;Dong Y;Wang Y;Liu Z
豆荚破碎引起的种子传播是许多野生物种使用的一种繁殖形式。在普通箭舌豌豆（Vicia sativa L.）中，一种重要的自花授粉的一年生豆科牧草。然而，落荚是限制野豌豆田间繁殖和作为豆科饲料作物使用的最重要缺陷之一。为了更好地理解野豌豆荚破碎机制，我们使用高通量RNA测序来评估从541个野豌豆种质中筛选的易破碎和抗破碎野豌豆种质的荚腹缝合线转录组的全球变化。共检测到1，285个显著差异表达的unigenes（DEG），其中上调unigenes 575个，下调unigenes 710个。对1，285个DEG的基因本体和KEGG代谢富集途径的分析表明，编码与荚果脱落相关的细胞壁修饰和水解酶的22个DEG在易脱落的种质中高度表达。这些基因主要富集在“水解酶活性”、“细胞质”和“碳水化合物代谢过程”系统中。这些细胞壁修饰和水解酶基因包括β-葡萄糖苷酶和内切多聚半乳糖醛酸酶，它们共同作用以破坏果胶和纤维素的糖苷键，并促进豆荚腹缝中细胞壁的溶解和消失，使豆荚更容易破碎。本研究首次揭示了箭舌豌豆易裂和抗裂品种间基因转录水平的差异，为箭舌豌豆裂荚调控网络的鉴定和表征提供了有价值的信息。这一信息可能有助于未来的识别荚果破碎相关基因及其潜在的分子机制，在共同的箭舌豌豆。
The seed dispersion caused by pod shattering is a form of propagation used by many wild species. Loss of seeds from pod shattering is frequent in the common vetch (Vicia sativa L.), an important self-pollinating annual forage legume. However, pod shattering is one of the most important defects that limits the reproduction of the vetch in the field and the usage as a leguminous forage crop. To better understand the vetch pod shattering mechanism, we used high-throughput RNA sequencing to assess the global changes in the transcriptomes of the pod ventral sutures of shattering-susceptible and shattering-resistant vetch accessions screened from 541 vetch germplasms. A total of 1,285 significantly differentially expressed unigenes (DEGs) were detected, including 575 up-regulated unigenes and 710 down-regulated unigenes. Analyses of Gene Ontology and KEGG metabolic enrichment pathways of 1,285 DEGs indicated that 22 DEGs encoding cell wall modifications and hydrolases associated with pod shattering were highly expressed in shattering-susceptible accessions. These genes were mainly enriched in “hydrolase activity,” “cytoplasm,” and “carbohydrate metabolic process” systems. These cell wall modifications and hydrolases genes included β-glucosidase and endo-polygalacturonase, which work together to break down the glycosidic bonds of pectin and cellulose, and to promote the dissolution and disappearance of the cell wall in the ventral suture of the pod and make the pod more susceptible to shattering. We demonstrated the differences in gene transcription levels between the shattering-susceptible and shattering-resistant vetch accessions for the first time and our results provided valuable information for the identifying and characterizing of pod shattering regulation networks in vetch. This information may facilitate the future identification of pod shattering-related genes and their underlying molecular mechanisms in the common vetch.