Defence mechanisms against insecticides temephos and diflubenzuron in the mosquito Aedes caspius:: the P-glycoprotein efflux pumps
Defence mechanisms against insecticides temephos and diflubenzuron in the mosquito Aedes caspius:: the P-glycoprotein efflux pumps
复制标题
DOI：
10.1111/j.1365-2915.2008.00712.x
复制
发表时间：
2008-03-01
影响因子：
1.9
通讯作者：
Urbanelli, S.
中科院分区：
文献类型：
--
作者：
Porretta, D.;Gargani, M.;Urbanelli, S.
P-糖蛋白（P-gps）是广泛存在于原核和真核生物细胞中的外排转运蛋白，其作用是释放多种结构不同的有毒化合物。研究了P-gps在蚊体内对杀虫双和灭幼脲的防御中的可能作用（双翅目：蚊科），并分离编码P-gp转运蛋白的基因组DNA序列，为将来研究编码P-gp转运蛋白的基因的表达和表征提供分子工具。用单独的杀虫剂和与亚致死剂量的P-gp抑制剂维拉帕米联合处理蚊子幼虫。P-GPS的抑制作用使替美磷和灭幼脲的LD 50值分别降低了3.5和16.4倍，表明P-GPS可能参与了杀虫剂的防御。使用基于聚合酶链反应（PCR）的方法，分离出481-bp的序列。推测的核苷酸序列与已知的P-gps的C-末端序列显示出高度的同源性。从Ae.中分离和鉴定一个推定的P-gp序列。caspius是对多药转运蛋白在该物种防御杀虫剂中所起作用的分子理解的第一步。这一知识可能开辟了一条新的控制策略的基础上抑制害虫防御。讨论了抑制外排泵对提高杀虫剂性能的有益影响。
P-glycoproteins (P-gps) are efflux transporters found in cells of a broad range of both procaryotic and eukaryotic taxa, whose action is to relieve the cells of multiple, structurally dissimilar, toxic compounds. The possible role of P-gps in defence against the insecticides temephos and diflubenzuron was investigated in the mosquito Aedes caspius (Pallas), also known as Ochlerotatus (Aedes) caspius (Diptera: Culicidae), and the genomic DNA sequences encoding for P-gp transporters were isolated to provide molecular instruments for future research into the expression and characterization of genes codifying for P-gps in this mosquito species.Mosquito larvae were treated with insecticides alone and in conjunction with a sublethal dose of the P-gp inhibitor verapamil. The inhibition of P-gps reduced the LD 50 values of temephos and diflubenzuron by factors of 3.5 and 16.4, respectively, suggesting the potential involvement of P-gps in insecticide defence. Using a polymerase chain reaction (PCR)-based approach, a 481-bp sequence was isolated. The inferred nucleotide sequence shows high homology with the C-terminal sequence of known P-gps. The isolation and characterization of a putative P-gp sequence from Ae. caspius is the first step towards a better molecular understanding of the role played by multidrug transporters in the defence against insecticides in this species. This knowledge may open the way to a novel control strategy based on the inhibition of pest defences. The beneficial consequences of the inhibition of efflux pumps in improving insecticide performance are discussed.