Diallyl disulfide from garlic oil inhibits Pseudomonas aeruginosa virulence factors by inactivating key quorum sensing genes
Diallyl disulfide from garlic oil inhibits Pseudomonas aeruginosa virulence factors by inactivating key quorum sensing genes
复制标题
大蒜油中的二烯丙基二硫化物通过抑制关键群体感应基因的转录来抑制铜绿假单胞菌毒力因子。
DOI：
10.1007/s00253-018-9175-2
复制
发表时间：
2018-09-01
影响因子：
5
通讯作者：
Huang, Xiao-Mo
中科院分区：
文献类型：
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作者：
Li, Wen-Ru;Ma, Yong-Kai;Huang, Xiao-Mo
大蒜油可以干扰条件致病菌铜绿假单胞菌的群体感应(QS)通路；然而，这种作用的潜在机制尚不清楚。二烯丙基二硫化物(DADS)是大蒜油中含量最丰富的含硫化合物之一。本研究研究了DADS对铜绿假单胞菌PAO1的生长、毒力因子(弹性酶、绿青素、生物膜和聚集运动)和必需基因表达的影响，特别是它们对QS和毒力的影响。1.28 mg/mLDADS对铜绿假单胞菌PAO1的生长没有影响，但它降低了弹性酶和绿青素的产量、生物膜的形成和集群运动。这些现象中的每一个都受到铜绿假单胞菌PAO1的三个QS系统(LAS、RHL和PQS)的调节。实时荧光定量聚合酶链式反应显示，在这三个系统中，DADS下调了几个重要的QS基因(LASI、LASR、RHLI、RHLR、PqsA和PqsR)的转录水平。此外，QS调控的毒力基因转录水平也下调。LasB基因编码LasB弹性酶，受LAS、RHL和PQS系统的共同调控，因此这三个系统中基因的下调进一步下调了LasB。此外，phzM(编码绿青素)、pslB(负责生物膜基质多糖的产生)和chic(编码几丁质酶)被LasR正激活，而LasR转录的降低进一步下调了phzM、pslB和chic的转录。因此，DADS通过在三个不同的QS系统中失活关键基因的转录来抑制铜绿假单胞菌PAO1毒力因子。
Garlic oil can disrupt the quorum sensing (QS) pathways of the opportunistic pathogen Pseudomonas aeruginosa; however, the underlying mechanisms for this effect are unclear. Diallyl disulfide (DADS) is one of the most abundant sulfur-containing compounds in garlic oil. This study investigated the effects of DADS on the growth, virulence factor production (elastase, pyocyanin, biofilm, and swarming motility), and essential gene expression of P. aeruginosa PAO1, particularly as they apply to QS and virulence. DADS at 1.28 mg/mL did not affect P. aeruginosa PAO1 growth, although it decreased elastase and pyocyanin production, biofilm formation, and swarming motility. Each of these phenomena is regulated by the three QS systems of P. aeruginosa PAO1 (las, rhl, and pqs). Real-time q-PCR revealed that DADS down-regulated the transcription levels of several important QS genes (lasI, lasR, rhlI, rhlR, pqsA, and pqsR) in the three systems. Furthermore, the transcription levels of QS-regulated virulence genes were also down-regulated. The lasB gene, encoding LasB elastase, is co-regulated by the las, rhl, and pqs systems, and thus the down-regulation of genes across the three systems further down-regulated lasB. Additionally, phzM (encoding pyocyanin), pslB (responsible for the production of a biofilm matrix polysaccharide), and chiC (encoding chitinase) were positively activated by LasR, and a decrease in lasR transcription further down-regulated the transcription of phzM, pslB, and chiC. Hence, DADS inhibits P. aeruginosa PAO1 virulence factors by inactivating the transcription of key genes across three different QS systems.