[Application of whole genome sequencing technology and bioinformatics analysis in antimicrobial resistance researches].
[Application of whole genome sequencing technology and bioinformatics analysis in antimicrobial resistance researches].
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DOI：
10.13345/j.cjb.180350
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发表时间：
2019-04-25
期刊：
影响因子：
--
通讯作者：
Wang, Shaolin
中科院分区：
文献类型：
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作者：
Shen, Yingbo;Shi, Xiaomin;Wang, Shaolin
抗菌素耐药性的出现和蔓延已成为一个严重的全球性问题。细菌特性，如抗菌素耐药基因、毒力相关基因、质粒类型、不同菌株之间的系统发育关系等，是解开抗菌素耐药性发生和传播的关键。然而，传统技术如聚合酶链式反应和脉冲场凝胶电泳法的准确性和效率不足以揭开耐药之谜。近年来，全基因组测序和高通量生物信息学分析已成功地应用于耐药性研究，帮助科学家快速获得耐药细菌的性质，更准确地描绘不同菌株之间的进化关系。因此，本研究旨在系统介绍全基因组测序技术的最新进展，包括文库制备、平台测序、数据分析的不同方法和特点，以及该技术在耐药研究中的最新应用。我们希望这篇综述能够为耐药研究提供更全面的全基因组测序和生物信息学分析的知识。
The emergence and spread of antimicrobial resistance has become a serious global issue. Bacterial characteristics, such as antimicrobial resistance genes, virulence-associated genes, plasmid types, and phylogenetic relationship among different strains, are the keys to unravel the occurrence and dissemination of antimicrobial resistance. However, the accuracy and efficiency of the traditional techniques, such as polymerase chain reaction and pulsed field gel electrophoresis is insufficient to underlying the mystery of antimicrobial resistance. Recently, the whole genome sequencing and high-throughput bioinformatics analysis have been successfully used in antimicrobial resistance studies, helping scientists to obtain the nature of antimicrobial resistance bacteria quickly, and more precisely to paint the evolutionary relationship among different strains. Therefore, in this study, we aim to systematically introduce the recent development of whole genome sequencing analysis, including different methods and corresponding characteristics of library preparation, platform sequencing, data analysis, and the latest application of the technology in the antimicrobial resistance research. We hope that this review can provide more comprehensive knowledge about whole genome sequencing and bioinformatic analysis for antimicrobial resistance research.