Stygiolobus rod-shaped virus and the interplay of crenarchaeal rudiviruses with the CRISPR antiviral system
Stygiolobus rod-shaped virus and the interplay of crenarchaeal rudiviruses with the CRISPR antiviral system
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DOI：
10.1128/jb.00795-08
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发表时间：
2008-10-01
影响因子：
3.2
通讯作者：
Prangishvili, David
中科院分区：
文献类型：
--
作者：
Vestergaard, Gisle;Shah, Shiraz A.;Prangishvili, David
从葡萄牙亚速尔群岛的一个温泉中分离出一种新鉴定的古菌鲁迪病毒斯蒂吉洛布斯杆状病毒（SRV），它感染超嗜热斯蒂吉洛布斯物种。其病毒体呈杆状，大小为 702 (+/- 50) x 22 (+/- 3) nm，无包膜，每个末端带有三个尾纤维。线性双链 DNA 基因组包含 28,096 bp 和 1,030 bp 的反向末端重复序列。 SRV 显示出与从冰岛和意大利的热酸性温泉中分离出的其他特征性 rudiviruses 硫化叶菌棒状病毒 1 (SIRV1)、SIRV2 和 Acidianus 棒状病毒 1 的形态和基因组相似性。研究表明，单一主要病毒结构蛋白可在体外产生与病毒粒子尺寸相似的长管状结构，我们估计病毒粒子构成了嵌入这种蛋白质结构中的单个超螺旋双链DNA。还鉴定了另外三种次要保守结构蛋白。具有指定功能的普遍存在的病毒蛋白包括糖基转移酶和S-腺苷甲硫氨酸依赖性甲基转移酶，以及霍利迪连接解离酶（一种与DNA复制有关的转录偶联解旋酶和核酸酶）。对参与病毒防御系统的已知天穹染色体 CRISPR 间隔序列与 rudiviral 基因组之间的匹配分析显示，3,042 个独特的嗜酸嗜热病毒间隔序列中，约 10% 与 rudiviral 基因组产生显着匹配，并偏向于高度保守的蛋白质基因，这与 rudivirus 在炎热嗜酸环境中的广泛存在一致。我们提出，保守病毒蛋白基因中常见的 12 bp 插入缺失可能是对宿主 CRISPR 防御系统存在的反应而产生的。
A newly characterized archaeal rudivirus Stygiolobus rod-shaped virus (SRV), which infects a hyperthermophilic Stygiolobus species, was isolated from a hot spring in the Azores, Portugal. Its virions are rod-shaped, 702 (+/- 50) by 22 (+/- 3) nm in size, and nonenveloped and carry three tail fibers at each terminus. The linear double-stranded DNA genome contains 28,096 bp and an inverted terminal repeat of 1,030 bp. The SRV shows morphological and genomic similarities to the other characterized rudiviruses Sulfolobus rod-shaped virus 1 (SIRV1), SIRV2, and Acidianus rod-shaped virus 1, isolated from hot acidic springs of Iceland and Italy. The single major rudiviral structural protein is shown to generate long tubular structures in vitro of similar dimensions to those of the virion, and we estimate that the virion constitutes a single, superhelical, double-stranded DNA embedded into such a protein structure. Three additional minor conserved structural proteins are also identified. Ubiquitous rudiviral proteins with assigned functions include glycosyl transferases and a S-adenosylmethionine- dependent methyltransferase, as well as a Holliday junction resolvase, a transcriptionally coupled helicase and nuclease implicated in DNA replication. Analysis of matches between known crenarchaeal chromosomal CRISPR spacer sequences, implicated in a viral defense system, and rudiviral genomes revealed that about 10% of the 3,042 unique acidothermophile spacers yield significant matches to rudiviral genomes, with a bias to highly conserved protein genes, consistent with the widespread presence of rudiviruses in hot acidophilic environments. We propose that the 12-bp indels which are commonly found in conserved rudiviral protein genes may be generated as a reaction to the presence of the host CRISPR defense system.