Clinical implications and characteristics of factor forkhead box protein 3 in gastric cancer.
Clinical implications and characteristics of factor forkhead box protein 3 in gastric cancer.
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DOI：
10.3892/etm.2011.264
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发表时间：
2011-07
影响因子：
2.7
通讯作者：
Chang-li Jiang;Weihua Wang;Wei Yan;Yuhai Zhang;Jiangtao Yang;Song Zhang;Cun Zhang;Wei Zhang;W. Han;Junzhi Wang;Ying-qi Zhang
中科院分区：
文献类型：
--
作者：
Chang-li Jiang;Weihua Wang;Wei Yan;Yuhai Zhang;Jiangtao Yang;Song Zhang;Cun Zhang;Wei Zhang;W. Han;Junzhi Wang;Ying-qi Zhang
转录因子叉头盒蛋白3（FOXP 3）是天然存在的调节性T细胞（Treg）的特异性标志物。最近，各种报告表明，FOXP 3可能代表了癌细胞中的肿瘤逃逸机制，除了它在Tumor中的作用。在本研究中，FOXP 3的临床和生物学特性进行了评估在人类胃癌。分析FOXP 3在胃癌细胞系中的表达和定位，以评价其细胞生物学特性。采用免疫组化（IHC）法对胃癌组织切片进行染色，分析FOXP 3表达与胃癌分化程度的关系，以明确FOXP 3在胃癌中的临床特征。FOXP 3 mRNA和蛋白在4种胃癌细胞系（AGS、SGC-7901、MKN-28和MKN-45）中均有表达。胃癌切片的IHC显示，超过56%的胃癌显示细胞核或细胞质FOXP 3染色。胃癌组织分化程度与FOXP 3表达强度呈线性关系。免疫组化和共聚焦显微镜分析显示，FOXP 3在组织和细胞系中主要表达于肿瘤细胞核。因此，FOXP 3核染色可能与肿瘤分化不良的风险相关。除淋巴细胞外，在正常胃组织和癌旁组织中未观察到FOXP 3染色。FOXP 3在胃癌组织中的高表达是研究肿瘤分化和免疫逃逸的重要发现。这一机制提供了对胃癌的进一步理解，并提出了一种新的治疗策略。
Transcription factor forkhead box protein 3 (FOXP3) is a specific marker of naturally occurring regulatory T cells (Tregs). Recently, various reports have suggested that FOXP3 may represent a tumor escape mechanism in cancer cells apart from its roles in Tregs. In the present study, the clinical and biological characteristics of FOXP3 were evaluated in human gastric cancer. The expression and localization of FOXP3 in gastric cancer cell lines was analyzed to evaluate its cellular biological features. Sections of human gastric cancer specimens were stained using immunohistochemistry (IHC) to assess the relationship between FOXP3 expression and tumor differentiation, in order to identify its clinical characteristics in gastric cancer. Expression of FOXP3 mRNA and protein was found in four gastric cancer cell lines (AGS, SGC-7901, MKN-28 and MKN-45). IHC of the gastric cancer sections revealed that more than 56% of gastric cancers displayed nuclear or cytoplasmic FOXP3 staining. Furthermore, a linear relationship between the differentiation of the gastric cancer tissues and FOXP3 expression intensity was shown. IHC and confocal analysis showed that the expression of FOXP3 was mainly present in the nucleus of tumor cells in the tissues and cell lines. Thus, FOXP3 nuclear staining may be associated with the risk of poor tumor differentiation. Apart from the lymphocytes, no FOXP3 staining was noted in the normal gastric tissues and para-tumor tissues. The high frequency of FOXP3 expression in gastric cancer tissue is a significant finding in the investigation of tumor differentiation and immune escape. This mechanism provides a further understanding of gastric cancer and a novel therapeutic strategy is presented.