Epithelial-Cell-Derived Extracellular Vesicles in Pathophysiology of Epithelial Injury and Repair in Chronic Rhinosinusitis: Connecting Immunology in Research Lab to Biomarkers in Clinics.
Epithelial-Cell-Derived Extracellular Vesicles in Pathophysiology of Epithelial Injury and Repair in Chronic Rhinosinusitis: Connecting Immunology in Research Lab to Biomarkers in Clinics.
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DOI：
10.3390/ijms222111709
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发表时间：
2021-10-28
影响因子：
5.6
通讯作者：
Schleimer RP
中科院分区：
文献类型：
--
作者：
Takahashi T;Schleimer RP
在慢性鼻窦炎（CRS）鼻粘膜中观察到的异常上皮-间质转化（EMT）诱导的基底细胞引起的上皮屏障破坏和上皮修复失败被推测在疾病病理生理学中起重要作用。微粒（MP）是一类由活化或凋亡细胞的质膜出芽或脱落而释放的细胞外囊泡（EV）。MP在鼻灌洗液（NLF）中被检测到，并且现在作为潜在的生物标志物受到关注，以评估患有鼻窦疾病的受试者的鼻粘膜中的免疫细胞活化和结构细胞损伤的程度。存在三种类型的上皮细胞来源的MP，其通过其表面上不同上皮特异性标志物的表达来定义：EpCAM、E-钙粘蛋白和整合素β6（ITGB 6）。当这些标志物在也携带典型EMT/间充质标志物的MP上时，（Snail（SNAI 1）; Slug（SNAI 2）; α-平滑肌肌动蛋白（αSMA，ACTA 2））或促凝血和抗凝分子（组织因子（TF）;组织纤溶酶原激活物（tPA）;纤溶酶原激活物抑制剂-1（派-1）），它们提供了关于上皮活化对EMT的作用或潜在疾病中凝血调节的见解。在这篇综述中，我们讨论了上皮MP作为研究工具的潜力，以评估在实验室中的CRS患者的鼻粘膜的状态，以及在临床上的CRS的管理和治疗的生物标志物。
Epithelial barrier disruption and failure of epithelial repair by aberrant epithelial-mesenchymal transition (EMT)-induced basal cells observed in nasal mucosa of chronic rhinosinusitis (CRS) are speculated to play important roles in disease pathophysiology. Microparticles (MPs) are a type of extracellular vesicle (EV) released by budding or shedding from the plasma membrane of activated or apoptotic cells. MPs are detected in nasal lavage fluids (NLFs) and are now receiving attention as potential biomarkers to evaluate the degree of activation of immune cells and injury of structural cells in nasal mucosa of subjects with sinus disease. There are three types of epithelial-cell-derived MPs, which are defined by the expression of different epithelial specific markers on their surface: EpCAM, E-cadherin, and integrin β6 (ITGB6). When these markers are on MPs that are also carrying canonical EMT/mesenchymal markers (Snail (SNAI1); Slug (SNAI2); alpha-smooth muscle actin (αSMA, ACTA2)) or pro- and anti-coagulant molecules (tissue factor (TF); tissue plasminogen activator (tPA); plasminogen activator inhibitor-1 (PAI-1)), they provide insight as to the roles of epithelial activation for EMT or regulation of coagulation in the underlying disease. In this review, we discuss the potential of epithelial MPs as research tools to evaluate status of nasal mucosae of CRS patients in the lab, as well as biomarkers for management and treatment of CRS in the clinic.
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影响因子：
4.9
作者：
Dieker J;Hilbrands L;Thielen A;Dijkman H;Berden JH;van der Vlag J
通讯作者：
van der Vlag J
影响因子：
8
作者：
De Paoli, Silvia H.;Tegegn, Tseday Z.;Simak, Jan
通讯作者：
Simak, Jan
影响因子：
13.6
作者：
Borges, Fernanda T.;Melo, Sonia A.;Kalluri, Raghu
通讯作者：
Kalluri, Raghu
影响因子：
3.5
作者：
Chiva-Blanch, Gemma;Bratseth, Vibeke;Seljeflot, Ingebjorg
通讯作者：
Seljeflot, Ingebjorg
影响因子：
20.3
作者：
Dalli, Jesmond;Norling, Lucy V.;Perretti, Mauro
通讯作者：
Perretti, Mauro