Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice
Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice
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DOI：
10.1007/978-1-4939-1139-4_4
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
Groves, Andrew K.
中科院分区：
文献类型：
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作者：
Gridley, Thomas;Groves, Andrew K.
人类Notch信号传导的异常导致先天性和获得性缺陷和癌症。基因工程小鼠为研究哺乳动物系统中的Notch信号提供了最有效和最具成本效益的模型。在这里，我们回顾了各种类型的遗传模型，工具和策略，以研究Notch信号在小鼠中，并提供了它们的使用的例子。我们还提供了条件突变小鼠的育种策略的建议，和他莫昔芬管理的小鼠株表达诱导型Cre重组酶-雌激素受体融合蛋白的协议。
Aberrations of Notch signaling in humans cause both congenital and acquired defects and cancers. Genetically engineered mice provide the most efficient and cost-effective models to study Notch signaling in a mammalian system. Here, we review the various types of genetic models, tools, and strategies to study Notch signaling in mice, and provide examples of their use. We also provide advice on breeding strategies for conditional mutant mice, and a protocol for tamoxifen administration to mouse strains expressing inducible Cre recombinase-estrogen receptor fusion proteins.