Adaptive wide-field optical tomography
Adaptive wide-field optical tomography
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DOI：
10.1117/1.jbo.18.3.036006
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发表时间：
2013-03
影响因子：
3.5
通讯作者：
V. Venugopal;X. Intes
中科院分区：
文献类型：
--
作者：
V. Venugopal;X. Intes
抽象的。我们描述了一种宽场光学层析成像技术，它允许测量引导优化照明模式，以增强重建性能。激励模式的迭代优化旨在减小通过生物组织传输的光子的动态范围。它增加了用高光子计数收集的测量值的数量，从而产生具有改进的断层摄影信息的数据集。在此，这种成像技术应用于时间分辨荧光分子断层扫描的临床前研究。首先，这种方法的优点是测试在硅片上的研究，在一个合成的小动物模型的典型照明模式。第二，这种方法在断层成像的适用性进行了验证，在体外使用一个小动物体模与两个荧光毛细血管闭塞的高度吸收夹杂物。模拟研究表明，对于所考虑的所有模式，通过小动物模型的中心部分传输的信号都有所改善（约2个数量级）。在发射波长处的信号中的1.6个数量级的相应改善证明了该技术用于荧光分子断层扫描的适用性。与非优化照明相比，使用优化图案以更高分辨率成功区分和定位两个对象（± 1 mm误差）确立了使用该技术时重建性能的改善。
Abstract. We describe a wide-field optical tomography technique, which allows the measurement-guided optimization of illumination patterns for enhanced reconstruction performances. The iterative optimization of the excitation pattern aims at reducing the dynamic range in photons transmitted through biological tissue. It increases the number of measurements collected with high photon counts resulting in a dataset with improved tomographic information. Herein, this imaging technique is applied to time-resolved fluorescence molecular tomography for preclinical studies. First, the merit of this approach is tested by in silico studies in a synthetic small animal model for typical illumination patterns. Second, the applicability of this approach in tomographic imaging is validated in vitro using a small animal phantom with two fluorescent capillaries occluded by a highly absorbing inclusion. The simulation study demonstrates an improvement of signal transmitted (∼2 orders of magnitude) through the central portion of the small animal model for all patterns considered. A corresponding improvement in the signal at the emission wavelength by 1.6 orders of magnitude demonstrates the applicability of this technique for fluorescence molecular tomography. The successful discrimination and localization (∼1 mm error) of the two objects with higher resolution using the optimized patterns compared with nonoptimized illumination establishes the improvement in reconstruction performance when using this technique.