Hansenula polymorpha Pex19p Is Essential for the Formation of Functional Peroxisomal Membranes*
Hansenula polymorpha Pex19p Is Essential for the Formation of Functional Peroxisomal Membranes*
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汉逊酵母 Pex19p 对于功能性过氧化物酶体膜的形成至关重要*
DOI：
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发表时间：
2004
影响因子：
4.8
通讯作者：
I. J. van der Klei
中科院分区：
文献类型：
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作者：
Marleen Otzen;Uta Perband;Dongyuan Wang;Richard J. S. Baerends;W. Kunau;M. Veenhuis;I. J. van der Klei
我们克隆并鉴定了多形汉逊酵母 PEX19 基因。在用甲醇诱导的 pex19 破坏菌株 (Hppex19) 的细胞中，未检测到过氧化物酶体结构；过氧化物酶体基质蛋白在细胞质中积累，而过氧化物酶体膜蛋白（PMP）错误定位到细胞质（Pex3p）和线粒体（Pex14p）或强烈降低至不可检测的水平（Pex10p）。 Hppex19 细胞中过氧化物酶体形成的缺陷在很大程度上受到 HpPex3p 或由 Pex3p 和 GFP 的前 50 个 N 末端氨基酸组成的融合蛋白的过量产生而抑制。在这些细胞中，PMP 再次被正确分类为过氧化物酶体结构，该结构也含有过氧化物酶体基质蛋白。在酿酒酵母 pex19 细胞中，ScPex3p 的过量产生导致形成大量含有 PMP 但缺乏主要基质蛋白硫解酶的囊泡。综上所述，我们的数据与 Pex19p 在膜蛋白组装和功能中的功能一致。
We have cloned and characterized the Hansenula polymorpha PEX19 gene. In cells of a pex19 disruption strain (Hppex19), induced on methanol, peroxisome structures were not detectable; peroxisomal matrix proteins accumulated in the cytosol, whereas peroxisomal membrane proteins (PMPs) were mislocalized to the cytosol (Pex3p) and mitochondria (Pex14p) or strongly reduced to undetectable levels (Pex10p). The defect in peroxisome formation in Hppex19 cells was largely suppressed upon overproduction of HpPex3p or a fusion protein that consisted of the first 50 N-terminal amino acids of Pex3p and GFP. In these cells PMPs were again correctly sorted to peroxisomal structures, which also harbored peroxisomal matrix proteins. In Saccharomyces cerevisiae pex19 cells overproduction of ScPex3p led to the formation of numerous vesicles that contained PMPs but lacked the major matrix protein thiolase. Taken together, our data are consistent with a function of Pex19p in membrane protein assembly and function.
甲醇诱导的博伊丁假丝酵母过氧化物酶体的膜蛋白。
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Goodman,JM;Maher,J;Silver,PA;Pacifico,A;Sanders,D
通讯作者：
Sanders,D