A Novel Ferroptosis-Related lncRNA Signature for Prognosis Prediction in Patients with Papillary Renal Cell Carcinoma.
A Novel Ferroptosis-Related lncRNA Signature for Prognosis Prediction in Patients with Papillary Renal Cell Carcinoma.
复制标题
一种用于乳头状肾细胞癌患者预后预测的新型铁死亡相关 lncRNA 特征
DOI：
10.2147/ijgm.s341034
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发表时间：
2022
影响因子：
2.3
通讯作者：
Li D
中科院分区：
文献类型：
--
作者：
Dang R;Jin M;Nan J;Jiang X;He Z;Su F;Li D
乳头状肾细胞癌是一种常见的肾细胞癌。近年来的研究表明，铁凋亡与肿瘤的发生、发展有关。长链非编码RNA可作为独立的生物标志物用于多种肿瘤的诊断和预后。从癌症基因组图谱（TCGA）数据库中获得PRCC患者的基因表达谱和临床信息。Lasso惩罚考克斯回归和单变量考克斯回归分析用于模型构建。绘制Kaplan-Meier（K-M）和受试者工作特征（ROC）曲线，以验证预后特征的预测效果。比较高危组和低危组的免疫细胞浸润和免疫功能。同时进行化疗敏感性分析。我们构建了一个由15个铁凋亡相关lncRNA组成的预后标志。K-M曲线验证了预后信号的良好预测准确性（p < 0.001）。lncRNA特征的曲线下面积（AUC）为0.930，表现出稳健的预后能力。高风险组的免疫细胞浸润程度高于低风险组。低危组和高危组在炎症促进、副炎症和I型IFN应答方面存在显著差异（p < 0.01）。高危组CD 80、IDO 1、LAG 3等免疫检查点的表达水平明显高于低危组（p < 0.05）。化疗敏感性分析显示MNX 1-AS 1、ZFAS 1、MIR 4435 - 2 HG、ADAMTS 9-AS 1与部分化疗药物敏感性显著相关（p < 0.05）。我们证明了铁中毒相关lncRNA预后标记可能是PRCC的新生物标志物。
Papillary renal cell carcinoma (PRCC) is a common renal cell carcinoma. Recent studies have reported that ferroptosis is involved in the occurrence and development of tumors. Long non-coding RNAs can be used as independent biomarkers for the diagnosis and prognosis of a variety of tumors. Gene expression profile and clinical information of patients with PRCC were obtained from The Cancer Genome Atlas (TCGA) database. Lasso penalized Cox regression and univariate Cox regression analysis were utilized for model construction. The Kaplan–Meier (K-M) and receiver operating characteristic (ROC) curves were plotted to validate the predictive effect of the prognostic signature. Immune cell infiltration and immune function were compared between the high-risk and low-risk groups. Chemotherapy sensitivity analysis was also performed. We constructed a prognostic signature consisting of 15 ferroptosis-related lncRNAs. The K-M curves validated the fine predictive accuracy of the prognostic signature (p < 0.001). The area under the curve (AUC) of the lncRNA signature was 0.930, exhibiting robust prognostic capacity. The high-risk group had a greater degree of immune cell infiltration than the low-risk group. Significant differences in inflammation promotion, parainflammation, and type I IFN response were noted between the low-risk and high-risk groups (p < 0.01). The expression levels of immune checkpoints including CD80, IDO1, and LAG3 were significantly higher in the high-risk group than in the low-risk group (p < 0.05). Chemotherapy sensitivity analysis showed that MNX1-AS1, ZFAS1, MIR4435-2HG, and ADAMTS9-AS1 were significantly correlated with the sensitivity of some chemotherapy drugs (p < 0.05). We demonstrated that a ferroptosis-related lncRNA prognostic signature could be a novel biomarker for PRCC.