A novel enzyme-free and label-free fluorescence aptasensor for amplified detection of adenosine
A novel enzyme-free and label-free fluorescence aptasensor for amplified detection of adenosine
复制标题
一种新型无酶、无标记荧光适体传感器，用于腺苷的放大检测
DOI：
10.1016/j.bios.2012.12.059
复制
发表时间：
2013-06-15
影响因子：
12.6
通讯作者：
Wang, Lei
中科院分区：
文献类型：
--
作者：
Fu, Bo;Cao, Jichao;Wang, Lei
基于靶点催化发夹结构自组装技术，构建了一种新型的无酶无标记荧光适体，用于腺苷的放大检测。该适体传感器含有四条DNA链，称为适体催化链、抑制链、发夹结构H1和H2，它们部分互补。同时，H2的茎中部分隐藏着一个可以形成DNA G-四链体的序列。在没有腺苷的情况下，适体催化链被抑制，并且不能触发H1和H2之间的自组装。当加入腺苷时，适配体和腺苷的结合事件触发H1和H2之间的自组装，导致在H1-H2复合物的末端形成G-四链体。添加N-甲基中卟啉IX，它具有显着的结构选择性的G-四链体，产生无标记的荧光信号。在最佳条件下，我们可以检测腺苷低至6 μ M，通过监测荧光强度的变化。此外，由于适体对靶标的特异性识别能力，这种扩增的适体传感器显示出对腺苷相对于其类似物的高选择性。因此，所提出的适体传感器可以用作目标检测的简单和选择性的平台。(C)2013爱思唯尔有限公司版权所有。
A novel enzyme-free and label-free fluorescence aptasensor based on target-catalyzed hairpin self-assembly is developed for amplified detection of adenosine. This aptasensor contains four DNA strands termed as aptamer-catalysis strand, inhibit strand, hairpin structures H1 and H2 which are partially complementary. Meanwhile, a sequence that can form DNA G-quadruplex is partly hidden in the stem of H2. In the absence of adenosine, aptamer-catalysis strand is inhibited, and cannot trigger the self-assembly between H1 and H2. Upon the addition of adenosine, the binding event of aptamer and adenosine triggers the self-assembly between H1 and H2, resulting in the formation of G-quadruplex at the end of H1-H2 complex. The addition of N-methyl mesoporphyrin IX, which has a pronounced structural selectivity for G-quadruplex, generates label-free fluorescence signal. In the optimum conditions, we could detect adenosine as low as 6 mu M by monitoring the change in fluorescence intensity. Furthermore, this amplified aptasensor shows high selectivity toward adenosine against its analogs due to the specific recognition ability of the aptamer for the target. Thus, the proposed aptasensor could be used as a simple and selective platform for target detection. (C) 2013 Elsevier B.V. All rights reserved.