Correlation of bromodeoxyuridine (BRDU) labeling of breast carcinoma cells with mitotic figure content and tumor grade.
Correlation of bromodeoxyuridine (BRDU) labeling of breast carcinoma cells with mitotic figure content and tumor grade.
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乳腺癌细胞的溴脱氧尿苷（BRDU）标记与有丝分裂象含量和肿瘤分级的相关性。
DOI：
10.1097/00000478-199310000-00003
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Smith,HS
中科院分区：
文献类型：
--
作者：
Weidner,N;Moore2nd,DH;Ljung,BM;Waldman,FM;Goodson3rd,WH;Mayall,B;Chew,K;Smith,HS
乳腺癌患者的肿瘤增殖与生存率呈负相关。用溴脱氧尿苷（BRDU）标记肿瘤细胞与用[3 H]胸苷（目前测量肿瘤S期的“金标准”）观察到的高度相关。然而，BRDU标记与核分裂相含量和肿瘤分级的关系仍不完全确定。为了确定这一点，我们在体内用BRDU标记了55例乳腺癌，并将结果与有丝分裂像内容相关联。BRDU标记指数为BRDU阳性细胞数/2，000个肿瘤细胞，核分裂像指数为每1，000个肿瘤细胞中的核分裂像数，核分裂像计数为每10个高倍视野中的核分裂像数。BRDU标记也与肿瘤分级相关（ScarffBloomRichardson）。BRDU标记指数与有丝分裂像指数（r= 0.814，p= 7.0±10− 14）、有丝分裂像计数（r= 0.725，p= 6.0±10− 10）和肿瘤分级（r= 0.68，p= 1.1±10− 8）高度相关。BRDU标记与有丝分裂像含量的相关性足够强，表明非常仔细测量的有丝分裂像指数提供了相当于BRDU标记指数的肿瘤生长分数的估计。此外，方差分析表明，有丝分裂像指数是两倍的有丝分裂像计数估计BRDU标记的精确度，因此是一个更准确的肿瘤增殖的措施。此外，有丝分裂像指数的测量结果与BRDU标记的测量结果一样精确。然而，哪种方法是估计肿瘤增殖率的最佳方法仍不清楚。进一步的研究表明。
Tumor proliferation is inversely associated with survival in patients with breast carcinoma. Labeling of tumor cells with bromodeoxyuridine (BRDU) correlates highly with that seen with [3 H] thymidine, the current “gold standard” for measuring tumor S-phase. However, the relationship of BRDU labeling to mitotic figure content and tumor grade remains incompletely defined. To determine this, we labeled 55 breast carcinomas with BRDU in vivo and correlated the results with mitotic figure content. The BRDU labeling index was the number of BRDU-positive cells/2, 000 tumor cells, the mitotic figure index was the number of mitotic figures per 1, 000 tumor cells, and the mitotic figure count was the number of mitotic figures per 10 high-powered fields. BRDU labeling was also correlated with tumor grade (ScarffBloomRichardson). The BRDU labeling index correlated highly with the mitotic figure index (r= 0.814, p= 7.0±10− 14), mitotic figure count (r= 0.725, p= 6.0±10− 10), and tumor grade (r= 0.68, p= 1.1±10− 8). The correlation of BRDU labeling with mitotic figure content was strong enough to suggest that a very carefully measured mitotic figure index provides an estimate of tumor growth fraction equivalent to the BRDU labeling index. Also, analysis of variance showed that the mitotic figure index was twice as precise as the mitotic figure count in estimating BRDU labeling, and thus was a more accurate measure of tumor proliferation. Moreover, measurements made by the mitotic figure index were as precise as those made by BRDU labeling. However, which method is optimal for estimating tumor proliferation rate remains unclear. Further studies are indicated.