Deletion of microRNA miR-223 increases Langerhans cell cross-presentation.
Deletion of microRNA miR-223 increases Langerhans cell cross-presentation.
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DOI：
10.1016/j.biocel.2012.11.004
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发表时间：
2013-02
影响因子：
4
通讯作者：
Zhou, Li
中科院分区：
文献类型：
--
作者：
Mi, Qing-Sheng;Xu, Yingping;Wang, He;Qi, Rui-Qun;Dong, Zheng;Zhou, Li
朗格汉斯细胞（LCs）是调节皮肤免疫的皮肤驻留树突状细胞。MicroRNAs （miRNAs）是控制多种细胞类型生物功能的关键调控因子。所有mirna的缺失会中断表皮LCs的稳态和功能。然而，单个mirna在调节LC发育和功能中的作用仍然是完全未知的。MiRNA miR-233尤其在髓系室中表达。在这里，我们报道了miR-223在新鲜分离的表皮LC中高表达，并使用miR-223敲除（KO）小鼠测试了miR-223是否调节LC的发育和功能。我们发现，在miR-223KO和野生型小鼠中，LCs的数量、成熟、迁移和吞噬能力是相当的。然而，缺乏miR-223显著增加了LCs介导的抗原特异性CD8+ T细胞在体内和体外的增殖，而来自KO和WT小鼠的LCs对抗原特异性CD4+ T细胞的刺激相当。我们的数据表明，miR-223负调控LC交叉呈现，但可能不是正常LC稳态和发育所必需的。
Langerhans cells (LCs) are skin-residential dendritic cells that regulate skin immunity. MicroRNAs (miRNAs) are key regulators in the control of biological functions in a variety of cell types. Deletion of all miRNAs interrupts the homeostasis and function of epidermal LCs. However, the roles of individual miRNAs in regulating LC development and function are still completely unknown. MiRNA miR-233 is especially expressed in the myeloid compartment. Here, we reported that miR-223 is highly expressed in freshly isolated epidermal LCs, and tested whether miR-223 regulates LC development and function using miR-223 knockout (KO) mice. We found that the number, maturation, migration and phagocytic capacity of LCs were comparable between miR-223KO and wild-type mice. However, lack of miR-223 significantly increases LCs-mediated antigen-specific CD8+ T cell proliferation in vivo and in vitro, while LCs from KO and WT mice showed comparable stimulation for antigen-specific CD4+ T cells. Our data suggest that miR-223 negatively regulates LC cross-presentation, but may not be required for normal LC homeostasis and development.
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DOI：
10.1084/jem.20061692
发表时间：
2006-10-30
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Cobb BS;Hertweck A;Smith J;O'Connor E;Graf D;Cook T;Smale ST;Sakaguchi S;Livesey FJ;Fisher AG;Merkenschlager M
通讯作者：
Merkenschlager M
影响因子：
64.8
作者：
Johnnidis, Jonathan B.;Harris, Marian H.;Camargo, Fernando D.
通讯作者：
Camargo, Fernando D.
影响因子：
5.5
作者：
Vennegaard, Marie T.;Bonefeld, Charlotte M.;Skov, Lone
通讯作者：
Skov, Lone
影响因子：
64.5
作者：
Lewis, BP;Shih, IH;Burge, CB
通讯作者：
Burge, CB
影响因子：
20.3
作者：
Heinz, LX;Platzer, B;Strobl, H
通讯作者：
Strobl, H